Evaluating Stress Granules in Pancreatic Cancer In Vitro and In Vivo.
Evaluating Stress Granules in Pancreatic Cancer In Vitro and In Vivo.
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体外和体内评估胰腺癌的应激颗粒。
DOI：
10.1007/978-1-4939-8879-2_17
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Grabocka,Elda
中科院分区：
文献类型：
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作者：
Sim,Edward;Irollo,Elena;Grabocka,Elda
应激颗粒是一种非膜性细胞器，其功能是应激适应机制。我们最近发现，应激颗粒在应激下被突变的KRAS胰腺癌细胞动员，以增强肿瘤适应性和存活率。在这一章中，我们概述了一种方法，用于诱导，检测和定量胰腺癌细胞在体外和体内的应激颗粒。这种方法可以用来更好地了解驱动应激颗粒形成的机制及其在胰腺肿瘤发生中的作用。
Stress granules are nonmembranous organelles that function as a stress-adaptation mechanism. We have recently shown that stress granules are mobilized by mutant KRAS pancreatic cancer cells under stress to enhance tumor fitness and survival. In this chapter, we outline a method for inducing, detecting, and quantifying stress granules in pancreatic cancer cells in vitro and in vivo. This method can be utilized to better understand the mechanisms driving stress granule formation and their role in pancreatic tumorigenesis.