Generation and characterization of the blood transcriptome of Macaca thibetana and comparative analysis with M-mulatta
Generation and characterization of the blood transcriptome of Macaca thibetana and comparative analysis with M-mulatta
复制标题
西藏猕猴血液转录组的生成和表征以及与 M-mulatta 的比较分析
DOI：
10.1039/c6mb00771f
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发表时间：
2017-06-01
影响因子：
--
通讯作者：
Li, Jing
中科院分区：
文献类型：
--
作者：
Li, Peng;Du, Lianming;Li, Jing
转录组图谱为基因组研究提供了大量的转录本序列数据集，特别是在那些没有已发表的准确基因组数据的生物中。藏酋猴（Macaca thibetana）通常被认为是中国特有的物种，并且是当今生物医学研究中的一种重要动物。在本研究中，我们报道了来自三只藏酋猴的血液转录组的从头组装和特征分析，并且我们还对恒河猴（Macaca mulatta）的血液转录组进行了测序以作比较。利用RNA - seq技术，产生了1.38亿条藏酋猴转录组的测序读长。组装得到了327871个转录本，N50为1571bp。根据序列相似性搜索，在nr蛋白质数据库中有80317个（24.5%）转录本被注释。利用GO和KEGG分析对藏酋猴和恒河猴的所有转录本进行了功能分类和比较。这两个转录组在营养库活性的GO术语以及信号分子与相互作用、传染病、细胞生长与死亡和免疫系统的KEGG子类别方面存在差异。本研究中的转录组将为未来的功能和比较基因组研究，甚至是这种非人灵长类动物的生物学研究提供有价值的资源。
Transcriptome profiles provide a large transcript sequence data set for genomic study, particularly in organisms that have no accurate genome data published. The Tibetan macaque (Macaca thibetana) is commonly considered to be an endemic species to China and an important animal in biomedical research in the present day. In the present study, we report the de novo assembly and characterization of the blood transcriptome of the Tibetan macaque from three individuals, and we also sequenced the blood transcriptome of the rhesus macaque (Macaca mulatta) for comparison. Using RNA-seq technology, 138 million sequencing reads of the M. thibetana transcriptome were generated. The assembly included 327871 transcripts with an N50 of 1571 bp. According to the sequence similarity search, 80317 (24.5%) transcripts were annotated in the nr protein database. All transcripts from M. thibetana and M. mulatta were functionally classified and compared using GO and KEGG analyses. The two transcriptomes were different in the GO term of nutrient reservoir activity, and in the KEGG subcategories of signaling molecules and interaction, infectious diseases, cell growth and death, and immune system. The transcriptomes in this study would provide a valuable resource for future functional and comparative genomic studies, and even for biological studies of this non-human primate.