Down-Regulation of A20 mRNA Expression in Peripheral Blood Mononuclear Cells from Patients with Systemic Lupus Erythematosus
Down-Regulation of A20 mRNA Expression in Peripheral Blood Mononuclear Cells from Patients with Systemic Lupus Erythematosus
复制标题
系统性红斑狼疮患者外周血单个核细胞中 A20 mRNA 表达的下调
DOI：
10.1007/s10875-012-9764-2
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发表时间：
2012-12-01
影响因子：
9.1
通讯作者：
Shi, Yongyu
中科院分区：
文献类型：
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作者：
Li, Di;Wang, Lei;Shi, Yongyu
目的A20基因具有负性免疫调节功能，其SNP与SLE发病相关。但其在SLE患者免疫细胞中的表达水平尚不清楚。本研究旨在探讨A20基因表达与SLE发病的关系。方法采用实时荧光定量RT-PCR方法检测37例SLE患者和32例健康对照者外周血单个核细胞（PBMC）中A20 mRNA的表达。结果SLE患者PBMC中A20 mRNA的表达较健康对照者明显降低（p = 0.0133）。A20基因表达水平与SLE疾病活动指数（SLEDAI）（r =-0.4661，p =0.0036）和红细胞沉降率（ESR）（r =-0.5222，p =0.0009）呈负相关。结论SLE患者PBMC中A20基因表达不足可能参与了SLE的发病过程。
PurposeA20 gene functions in negative immunoregulation and its SNP is related to SLE disease. But its expression level in immune cells from SLE patients is still unclear. The aim of this study is to investigate whether the expression of A20 is associated with pathogenesis of SLE.MethodsReal-time transcription-polymerase chain reaction analysis (RT-PCR) was used to determine expression of A20 mRNA in peripheral blood mononuclear cells (PBMC) from 37 patients with SLE and 32 healthy controls.ResultsA20 expression was decreased in SLE patients compared with healthy controls (p= 0.0133). The expression level of A20 gene negatively correlated with the SLE disease activity index (SLEDAI) (r=−0.4661,p= 0.0036) and erythrocyte sedimentation rate (ESR) (r=−0.5222,p= 0.0009). Furthermore, SLE patients with nephritis had a lower expression of A20 than those without nephritis (p= 0.0188).ConclusionsOur results suggest that the insufficient expression of A20 gene in PBMC may take part in the pathogenesis of SLE disease.