Immune responses against Lewis Y tumor-associated carbohydrate antigen displayed densely on self-assembling nanocarriers
Immune responses against Lewis Y tumor-associated carbohydrate antigen displayed densely on self-assembling nanocarriers
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针对 Lewis Y 肿瘤相关碳水化合物抗原的免疫反应在自组装纳米载体上密集显示
DOI：
10.1039/c8ob01955j
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发表时间：
2018
影响因子：
3.2
通讯作者：
Kimura Shunsaku
中科院分区：
文献类型：
--
作者：
Yamazaki Yuji;Nambu Yukiko;Ohmae Masashi;Sugai Manabu;Kimura Shunsaku
研究了不同表面密度的纳米载体对Lewis y(LY)的免疫反应。A2B型两亲性多肽[LY-聚(肌氨酸)2-b-(L或D-亮氨酸-AIB)6]具有较高的表面密度。这两个两亲性多肽的等摩尔混合物形成了交错的平面片状分子集合体，密集地显示LY(G4)。G4单次免疫BALB/c小鼠可诱导产生抗LY-IgM。然而，抗LY-IgM的产量适中，明显少于另一种分子组件(G1)的两次给药，LY的平均表面密度是G4的1/4。此外，G4两次给药后产生的抗LY IgM抗体比一次给药后亲和力降低得更多。
Immune responses against Lewis y (LY) displayed on nanocarriers at different surface densities were studied. The high surface density of LY was obtained by the A2B-type amphiphilic polypeptides having LY at the two terminals [LY-poly(sarcosine)2-b-(L- or D-Leu-Aib)6]. The equimolar mixture of these two amphiphilic polypeptides formed interdigitated planar sheet-like molecular assemblies densely displaying LY (G4). G4 seemed to induce the anti-LY IgM upon immunization to BALB/c mice by only a single administration. However, the amount of anti-LY IgM produced was moderate and significantly less than that induced by two administrations of the other molecular assembly (G1) with the average surface density of LY at a 1/4 of that of G4. Further, the anti-LY IgM produced after two administrations of G4 lowered the avidity more than after one administration.