Comparative chloroplast genomics of series Sakawanum in genus Asarum (Aristolochiaceae) to develop single nucleotide polymorphisms (SNPs) and simple sequence repeat (SSR) markers
Comparative chloroplast genomics of series Sakawanum in genus Asarum (Aristolochiaceae) to develop single nucleotide polymorphisms (SNPs) and simple sequence repeat (SSR) markers
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比较细辛属（马兜铃科）Sakawanum 系列叶绿体基因组学，以开发单核苷酸多态性 (SNP) 和简单序列重复 (SSR) 标记
DOI：
10.1080/13416979.2018.1518649
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发表时间：
2018
影响因子：
1.5
通讯作者：
Setoguchi Hiroaki
中科院分区：
文献类型：
--
作者：
Takahashi Daiki;Sakaguchi Shota;Isagi Yuji;Setoguchi Hiroaki
马兜铃属（SeriesSakawanum, asarum，马兜铃科）由森林林下草本植物4个分类群组成，其花萼叶长在不同的分类单元上呈现地理梯度。为了了解这一系列的形成过程，有必要研究基于母系遗传的种子介导质体标记的进化史。利用焦磷酸测序技术，对两个物种(A. minamitanianumandA.；肋片)。此外，还开发出9个叶绿体单核苷酸多态性（snp）和11个简单序列重复（SSR）标记。这些标记大部分表现出种间变异，所有标记都表现出可转移性，而大多数标记在种群内的变异很小。这些叶绿体基因组和标记可用于进一步研究母系在塑造卡卡瓦人（seriesakawanuman）群体遗传结构中的贡献，以及其形态多样化的进化过程，包括典型的萼叶长度变化。此外，本研究还表明，比较叶绿体基因组分析方法适用于利用通用引物难以获得多态性数据的种群叶绿体标记。
SeriesSakawanum(genusAsarum, Aristolochiaceae) comprises four taxa of forest understory herbaceous plants, which exhibits a geographical cline of calyx lobe length across taxonomic units. To understand the formation process of this series, it is necessary to investigate the evolutionary history based on maternally inherited seed-mediated plastid markers. Using pyrosequencing technique, nearly complete chloroplast genomes of two species (A. minamitanianumandA. costatum) were obtained. Furthermore, nine polymorphic chloroplast single nucleotide polymorphisms (SNPs) and 11 simple sequence repeat (SSR) markers were developed. Most of these markers showed interspecific variations and all markers showed transferability to otherAsarumspecies, while most of markers showed little intra-population variations. These chloroplast genomes and markers can be applied in future studies investigating the contributions of the maternal lineage in shaping the population genetic structure of seriesSakawanumand the evolutionary processes underlying their morphological diversification including the characteristic calyx lobe length variation. Moreover, this study showed the applicability of comparative chloroplast genome analysis to develop the chloroplast markers for species group for which it is difficult to obtain polymorphism data using the universal primers.