SYNTHESIS OF ALPHA-2-MACROGLOBULIN IN HUMAN GINGIVA - A STUDY OF THE CONCENTRATION OF MACROGLOBULIN AND ALBUMIN IN GINGIVAL FLUID AND SERUM
SYNTHESIS OF ALPHA-2-MACROGLOBULIN IN HUMAN GINGIVA - A STUDY OF THE CONCENTRATION OF MACROGLOBULIN AND ALBUMIN IN GINGIVAL FLUID AND SERUM
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DOI：
10.1016/0003-9969(90)90107-l
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发表时间：
1990-01-01
影响因子：
3
通讯作者：
CIMASONI, G
中科院分区：
文献类型：
--
作者：
GIANNOPOULOU, C;DEFELICE, R;CIMASONI, G
对6名志愿者在菌斑堆积前、期间和10天后用滤纸条收集的牙周液中的白蛋白和α2-巨球蛋白进行了分析。在戒牙开始和10天后的静脉血样本中也检测了这两种蛋白质。白蛋白的分析采用散射比浊法，而α2-巨球蛋白的测定采用ELISA法。菌斑和牙龈指数以及牙周液流量在菌斑堆积期间显著增加，然后恢复到基线值。血液中这两种蛋白质的浓度保持在正常范围内。然而，在牙周液中，白蛋白的浓度在炎症高峰期增加了大约两倍，而α2-巨球蛋白的浓度增加了6倍。与完全由血浆衍生的白蛋白相比，α2-巨球蛋白进入牙龈沟的通道增强，这与炎症期间球蛋白的更大排泄一定是由于局部合成增加的概念一致。
Albumin and .alpha.2-macroglobulin were analysed in gingival fluid collected by filter paper strips from 6 volunteers before, during and after 10 days of dental plaque accumulation. The two proteins were also determined in samples of venous blood at the beginning of abstention from tooth brushing and 10 days later. A nephelometric technique was used for the analysis of albumin, but .alpha.2-macroglobulin was determined by ELISA. The plaque and gingival indices, as well as the flow of gingival fluid, increased significantly during the period of plaque accumulation and then returned to baseline values. The concentrations of the two proteins in blood remained within normal limits. In gingival fluid, however, the concentration of albumin increased about two-fold at the peak of inflammation, while that of .alpha.2-macroglobulin increased as much as 6 times. The enhanced passage of .alpha.2-macroglobulin into the gingival sulcus in comparison to the entirely plasma-derived albumin was consistent with the concept that the greater excretion of globulin during inflammation must be due to an increased local synthesis.