Marker-assisted selection to improve drought resistance in common bean
Marker-assisted selection to improve drought resistance in common bean
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DOI：
10.2135/cropsci1997.0011183x003700010008x
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发表时间：
1997-01-01
期刊：
影响因子：
2.3
通讯作者：
Kelly, JD
中科院分区：
文献类型：
--
作者：
Schneider, KA;Brothers, ME;Kelly, JD
标记辅助选择（MAS）方法的发展将促进诸如抗旱性等数量性状的育种，这种方法能够在早期几代中识别出高性能基因型。从1990年到1994年，在美国密歇根州和墨西哥的8个地点分别培养了2个重组自交系群体，在胁迫和非胁迫条件下鉴定了菜豆（Phaseolus vulgaris L）抗旱性的随机扩增多态性DNA （RAPD）标记。利用单因素方差分析和多元回归分析，在一个群体中鉴定出4个RAPD标记，在另一个群体中鉴定出5个RAPD标记，这些标记与胁迫下产量、非胁迫下产量和/或广泛环境下的几何平均产量一致且显著相关。为了测试MAS抗旱性的有效性，使用标记从两个极端选择基因型，并检查了来自三个地点的产量数据，以评估这些基于标记选择的有效性。在Sierra/AC1028群体中，标记辅助选择在重度胁迫下在密歇根州有效，在中度胁迫下在墨西哥无效。在Sierra/Lef-2RB群体中，用于MAS的5种RAPD标记在胁迫下提高了11%的产量，在非胁迫下提高了8%，而基于产量表现的常规选择未能提高产量，在Sierra/AC1028群体中，对常规选择的反应是常规选择的3倍。支持MAS的有效性与被检测性状的遗传力成反比的结论。
Breeding for a quantitative trait like drought resistance would be facilitated by the development of a method of marker-assisted selection (MAS) that is capable of identifying high performing genotypes in early generations, Two recombinant inbred populations were grown from 1990 to 1994 at eight locations in Michigan and Mexico under stress and nonstress conditions to identify random amplified polymorphic DNA (RAPD) markers associated with drought resistance in common bean (Phaseolus vulgaris L). Six-hundred random decamer primers were screened against the parents of the two populations, 50% of which were polymorphic, Seventy of these polymorphic primers were screened against each population, Using one-way analysis of variance and multiple regression, four RAPD markers were identified in one population and five in another that were consistently and significantly associated with yield under stress, yield under nonstress, and/or geometric mean yield across a broad range of environments, To test the effectiveness of MAS for drought resistance, markers were used to select genotypes from either extreme, Yield data from three locations were examined to evaluate the effectiveness of these marker-based selections. Marker-assisted selection in the Sierra/AC1028 population was found to be effective in Michigan under severe stress and ineffective in Mexico under moderate stress, The five RAPD markers used for MAS in the Sierra/Lef-2RB population improved performance 11% under stress and 8% under nonstress, whereas conventional selection based on yield performance failed to increase performance, response to conventional selection was three times greater in the Sierra/AC1028 population, supporting the conclusion that the effectiveness of MAS is inversely proportional to the heritability of the trait under examination.