Double heat shock more potently suppresses cleavage of fertilized willow minnow ( Gnathopogon caerulescens ) eggs than single heat‐shock treatment
Double heat shock more potently suppresses cleavage of fertilized willow minnow ( Gnathopogon caerulescens ) eggs than single heat‐shock treatment
复制标题
与单次热激处理相比，双重热激更有效地抑制受精柳鲦鱼（Gnathopogon caerulescens）卵的分裂
DOI：
10.1111/are.14225
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发表时间：
2019
影响因子：
2
通讯作者：
Hikaru
中科院分区：
文献类型：
--
作者：
Kobayashi;Toru and Fujii;Hikaru
本研究探讨了双重热休克处理(HST)抑制受精柳鱼卵裂的效果和最佳条件。此外，我们还对HST前后的细胞核和有丝分裂器进行了细胞学分析。第一次受精后25min和第二次受精后15min的四倍体发生率最高(45.4%)。在这组中，纺锤体在第二次HST完成后立即萎缩；随后，在第一个细胞周期的核和有丝分裂器中观察到三种不同的模式。在第一种模式中，一极消失，形成单极纺锤体；染色体无法分离并聚集在一极，在那里它们形成一个大核。在第二种模式中，极点分散或集中在核的一侧，导致染色体无法分离，形成单个核。在第三种模式中，第一次卵裂正常完成。因此，双HST可以通过消除一个中心体或将两个极点靠近而阻止第一次卵裂分裂，从而产生一个四倍体胚胎子集。
This study examined the effects and optimal conditions of double heat‐shock treatment (HST) for the suppression of cleavage in fertilized willow minnow (Gnathopogon caerulescens) eggs. In addition, we performed a cytological analysis of the nucleus and mitotic apparatus dynamics before and after HST. Tetraploidy occurred at the highest frequency (45.4%) when the first HST was delivered 25 min after fertilization and the second 15 min later. In this group, the spindle atrophied immediately after completion of the second HST; subsequently, three distinct patterns were observed in the nucleus and mitotic apparatus of the first cell cycle. In the first pattern, one pole disappeared and a monopolar spindle formed; chromosomes were unable to separate and accumulated at one pole where they formed a large nucleus. In the second pattern, the poles were dispersed or localized to one side of the nucleus, resulting in the failure of chromosomes to separate and the formation of a single nucleus. In the third pattern, the first cleavage was completed normally. Thus, double HST can block the first cleavage division by eliminating one centrosome or bringing two poles close together, giving rise to a subset of tetraploid embryos.