The cooperative induction of macrophage activation by fucoidan derived from Cladosiphon okamuranus and β-glucan derived from Saccharomyces cerevisiae
The cooperative induction of macrophage activation by fucoidan derived from Cladosiphon okamuranus and β-glucan derived from Saccharomyces cerevisiae
复制标题
DOI：
10.1016/j.bbrc.2019.06.010
复制
发表时间：
2019-08-13
影响因子：
3.1
通讯作者：
Tachikawa, Daisuke
中科院分区：
文献类型：
--
作者：
Miyazaki, Yoshiyuki;Iwaihara, Yuri;Tachikawa, Daisuke
本研究探讨了枝管藻岩藻聚糖硫酸酯对小鼠巨噬细胞样细胞系RAW 264的免疫刺激作用，以及与酵母菌β-葡聚糖（Zymosan）的功能关系。RAW 264细胞中一氧化氮（NO）和肿瘤坏死因子-α（TNF-α）的产生在10 μ g/mL岩藻依聚糖的存在下显著增强，并且岩藻依聚糖的刺激作用在与500 ng/mL酵母聚糖的组合处理中最大程度地增强，而任何TLR配体都没有这些作用。共聚焦显微镜分析表明，褐藻糖胶结合在细胞膜上，推测某些细胞表面分子作为褐藻糖胶的受体，因为细胞吞噬抑制剂细胞松弛素D不影响褐藻糖胶的免疫增强活性，而脂筏破坏剂甲基-β-环糊精（M β CD）则减弱了褐藻糖胶的免疫增强活性。此外，基于dectin-1敲低的RAW 264细胞的结果，揭示了酵母聚糖对岩藻依聚糖的免疫激活的累加效应被认为是由dectin-1介导的。以上结果表明，褐藻糖胶和某些β-葡聚糖通过相互作用的受体串扰，协同增强天然免疫细胞的活性。(C)2019爱思唯尔公司All rights reserved.
The present study investigated immune stimulatory effects of Cladosiphon okamuranus-derived fucoidan to activate murine macrophage-like cell line RAW264, and the functional relationship with zymosan, a Saccharomyces cerevisiae-derived beta-glucan. The production of nitric oxide (NO) and tumor necrosis factor-alpha (TNF-alpha) in RAW264 cells were remarkably enhanced in the presence of 10 mu g/mL fucoidan, and the stimulatory effects of fucoidan were maximally augmented in combinational treatment with 500 ng/mL zymosan, whereas any TLR ligands had no those effects. Confocal microscopic analyses suggested that fucoidan bound on plasma membrane, and it was estimated that some cell surface molecules acted as receptor for fucoidan because cytochalasin D, an inhibitor of phagocytosis, did not affect the immune enhancing activities, whereas methyl-beta-cyclodextrin (M beta CD), a general agent for disruption of lipid rafts, diminished that. Furthermore, it was revealed that the additive effects of zymosan on the immune activation with fucoidan was thought to be mediated by dectin-1 based on the results with dectin-1-knockdown RAW264 cells. All of results suggested that fucoidan and some kinds of beta-glucan would cooperatively reinforce the activity of innate immune cells via interactive receptor crosstalk. (C) 2019 Elsevier Inc. All rights reserved.