KINETIC INTERMEDIATES IN THE REACTIONS BETWEEN PEPTIDES AND PROTEINS OF MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-II
KINETIC INTERMEDIATES IN THE REACTIONS BETWEEN PEPTIDES AND PROTEINS OF MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-II
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DOI：
10.1073/pnas.91.19.8842
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发表时间：
1994-09-13
影响因子：
11.1
通讯作者：
MCCONNELL, HM
中科院分区：
文献类型：
--
作者：
BEESON, C;MCCONNELL, HM
The kinetics of the reactions between fluorescently labeled sperm whale myoglobin-(110-121) peptide and the murine major histocompatibility complex class II protein I-E(d) have been analyzed. The presence in solution of both short-and long-lived protein-peptide complexes is demonstrated by the biphasic dissociation of the myoglobin peptide from I-E(d). The formation of the long-lived terminal complex is preceded by a characteristic induction phase. It is shown that the initially formed complex of the myoglobin peptide and I-E(d) is a kinetic intermediate that undergoes a unimolecular reaction to form the terminal complex. Reactions between peptides and the class II proteins thus involve an intermediate structurally distinct from the terminal complex. The terminal complex presumably has a structure that is biologically active and similar to the published class II protein-peptide crystal structure.
