Regulation of Iodide Uptake in Placental Primary Cultures
Regulation of Iodide Uptake in Placental Primary Cultures
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DOI：
10.1159/000356847
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发表时间：
2013-01-01
影响因子：
4.7
通讯作者：
Smyth, P. P. A.
中科院分区：
文献类型：
--
作者：
Burns, R.;O'Herlihy, C.;Smyth, P. P. A.
背景资料：维持发育中胎儿充足的碘供应不仅取决于母体膳食碘摄入量，还取决于胎盘碘转运。本研究的目的是研究不同的妊娠相关激素对胎盘摄取放射性碘的影响，并确定碘转运蛋白的表达是否受激素孵育的影响。方法：从足月妊娠的胎盘中分离胎盘滋养层细胞，进行原代培养。将它们与17 β-雌二醇、催乳素、催产素、人绒毛膜促性腺激素（hCG）和孕酮单独或组合预孵育12 h，6 h后测量125 I摄取。从胎盘滋养层细胞中分离RNA，并使用碘化钠同向转运体（NIS）和pendrin（PDS）探针进行实时RT-PCR。结果如下：在与hCG（增加60%）、催产素（增加45%）和催乳素（增加32%）孵育后，观察到滋养层细胞摄取125 I的显著剂量反应增加（p < 0.01）。虽然单独使用孕酮（50-200 ng/ml）和17 β-雌二醇（1，000 - 15，000 pg/ml）在摄取方面没有显著差异，但当与催乳素或催产素结合时，它们促进了摄取的增加，所有四种激素的组合产生了最大的增加（82%）。与未处理的对照细胞相比，增加的125 I摄取伴随着NIS mRNA的相应增量（比率1.52）。没有观察到PDS的表达水平显著增加。结论：妊娠相关激素，特别是催产素和hCG，具有促进胎盘碘摄取的作用，可保护胎儿免受碘缺乏。(C)2013欧洲甲状腺协会
Background: Maintenance of adequate iodide supply to the developing fetus is dependent not only on maternal dietary iodine intake but also on placental iodide transport. The objective of this study was to examine the effects of different pregnancy-associated hormones on the uptake of radioiodide by the placenta and to determine if iodide transporter expression is affected by hormone incubation. Methods: Primary cultures of placental trophoblast cells were established from placentas obtained at term from pre-labor caesarean sections. They were pre-incubated with 17 beta-estradiol, prolactin, oxytocin, human chorionic gonadotropin (hCG) and progesterone either singly or in combination over 12 h with 125 I uptake being measured after 6 h. RNA was isolated from placental trophoblasts and real-time RT-PCR performed using sodium iodide symporter (NIS) and pendrin (PDS) probes. Results: Significant dose response increments in 125 I uptake by trophoblast cells (p < 0.01) were observed following incubation with hCG (60% increase), oxytocin (45% increase) and prolactin (32% increase). Although progesterone (50-200 ng/ml) and 17 beta-estradiol (1,000-15,000 pg/ml) alone produced no significant differences in uptake, they facilitated increased uptake when combined with prolactin or oxytocin, with a combination of all four hormones producing the greatest increase (82%). Increased 125 I uptake was accompanied by corresponding increments in NIS mRNA (ratio 1.52) compared to untreated control cells. No significantly increased expression levels of PDS were observed. Conclusions: Pregnancy-associated hormones, particularly oxytocin and hCG, have a role in promoting placental iodide uptake which may protect the fetus against iodine deficiency. (C) 2013 European Thyroid Association