Electroimmunoassay. A new competitive protein-binding assay using antibody-sensitive electrodes.
Electroimmunoassay. A new competitive protein-binding assay using antibody-sensitive electrodes.
复制标题
电免疫测定。
DOI：
10.1016/s0006-3495(83)84284-2
复制
发表时间：
1983
影响因子：
3.4
通讯作者：
Williams,RL
中科院分区：
文献类型：
--
作者：
Connell,GR;Sanders,KM;Williams,RL
报道了一种新的技术，其中用于抗前列腺素E2抗血清的抗体敏感电极用于测量纳摩尔量的溶液相PGE 2。通过将阳离子选择性离子载体-半抗原（PGE 2）结合物掺入聚氯乙烯膜中来构建电极。测定固定钾梯度下的跨膜电位。抗PGE 2抗血清的加入以浓度依赖性方式改变膜电位。通过向含有抗血清的缓冲液中加入游离PGE 2，降低抗PGE 2抗体对膜电位的影响。利用这种技术，开发了竞争性蛋白结合测定，并在1至1，000 nM的浓度范围内生成了溶液相PGE 2的标准曲线。该试验对PGE 2具有相对特异性;在PGE 2的有效浓度范围内，PGD 2和PGF 2a对跨膜电位仅有轻微影响。
A novel technique is reported in which an antibody-sensitive electrode for anti-prostaglandin E2 antisera was used to measure solution-phase PGE2 in nanomolar quantities. The electrode was constructed by incorporating a cation-selective ionophore-hapten (PGE2) conjugate into a polyvinyl chloride membrane. Transmembrane potential in a fixed potassium gradient was measured. The addition of anti-PGE2 antisera changed membrane potential in a concentration-dependent manner. The effect of anti-PGE2 antibodies on membrane potential was decreased by adding free PGE2 to the buffer-containing antisera. With this technique a competitive protein-binding assay was developed, and standard curves for solution-phase PGE2 were generated over a concentration range of 1 to 1,000 nM. The assay was relatively specific for PGE2; PGD2 and PGF2a had only minor effects on transmembrane potential over the effective concentration range for PGE2.