Role of extracellular HSP72 in acute stress-induced potentiation of innate immunity in active rats
Role of extracellular HSP72 in acute stress-induced potentiation of innate immunity in active rats
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DOI：
10.1152/japplphysiol.00681.2002
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发表时间：
2003-01-01
影响因子：
3.3
通讯作者：
Fleshner, M
中科院分区：
文献类型：
--
作者：
Campisi, J;Fleshner, M
急性压力会损害后天免疫力，增强先天免疫力。最近的证据表明，应激对免疫措施的影响可以通过生物体的身体活动状态进行调节，并且细胞外热休克蛋白72（eHSP 72）有助于激活应激产生的先天免疫。因此，本研究调查了体力活动状态是否会影响应激暴露[不可避免的尾部休克应激（IS）]对先天免疫的免疫增强作用，以及eHSP 72反应的变化是否会发挥作用。成年雄性Fischer 344大鼠与移动的（体力活动）或非移动的（久坐）运行轮一起生活。6周后，大鼠暴露于IS或无应激。IS后立即对所有大鼠皮下注射活大肠杆菌。每天评估炎症，并在不同时间点测量血浆eHSP 72。暴露于IS的大鼠比无压力的大鼠更快地解决了炎症，但压力对恢复的有益影响在体力活动的大鼠中更大。IS后所有大鼠循环eHSP 72均增加。脾细胞收获从一个单独的队列的非应激大鼠培养的eHSP 72，一氧化氮和细胞因子进行了测量。体力活动的大鼠在体外对eHSP 72刺激的反应比久坐的大鼠具有更大的一氧化氮和细胞因子反应。因此，在细菌攻击+ IS暴露后，体力活动大鼠比久坐大鼠恢复得更快，并且表现出对eHSP 72活化的增强的细胞应答，这对于细菌恢复可能是重要的。
Acute stress can compromise acquired, and potentiate innate, immunity. Recent evidence suggests that the impact of stress on measures of immunity can be modulated by the physical activity status of the organism and that extracellular heat shock protein 72 (eHSP72) contributes to the activation of innate immunity produced by stress. Therefore, this study investigated whether physical activity status would impact the immunologically enhancing effects of stressor exposure [inescapable tail-shock stress (IS)] on innate immunity and whether changes in eHSP72 responses could play a role. Adult, male Fischer 344 rats lived with mobile (physically active) or immobile (sedentary) running wheels. After 6 wk, rats were exposed to IS or to no stress. Immediately after IS, all rats were injected subcutaneously with live Escherichia coli. Inflammation was assessed daily, and plasma eHSP72 was measured at various time points. Rats exposed to IS resolved their inflammation faster than nonstressed rats, but the beneficial impact of stress on recovery was greater in physically active rats. All rats had equal increases in circulating eHSP72 after IS. Splenocytes harvested from a separate cohort of nonstressed rats were cultured with eHSP72, and nitric oxide and cytokines were measured. Physically active rats responded to eHSP72 stimulation in vitro with a greater nitric oxide and cytokine response than sedentary rats. Thus physically active rats both recover faster than sedentary rats after bacterial challenge + IS exposure and demonstrate potentiated cellular responses to eHSP72 activation that could be important for bacterial recovery.