Reduced expression of N-Myc downstream-regulated gene 2 in human thyroid cancer
Reduced expression of N-Myc downstream-regulated gene 2 in human thyroid cancer
复制标题
人甲状腺癌中 N-Myc 下游调节基因 2 表达降低
DOI：
10.1186/1471-2407-8-303
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发表时间：
2008-10-22
期刊：
影响因子：
3.8
通讯作者：
Yao, Libo
中科院分区：
文献类型：
--
作者：
Zhao, Huadong;Zhang, Jian;Yao, Libo
NDRG 2（N-Myc downstream-regulated gene 2）是本实验室首次克隆的。已有研究表明NDRG 2在正常组织和肿瘤组织中表达差异。NDRG 2在多种肿瘤中表达下调或检测不到，过表达可抑制肿瘤细胞的增殖，并在细胞分化和肿瘤抑制中发挥重要作用。然而，它仍然不清楚whether NDRG 2参与甲状腺癌的发生。MethodsIn这项研究中，我们调查了人类NDRG 2在甲状腺腺瘤和癌的表达谱，通过检查组织从个人甲状腺腺瘤（n = 40）和癌（n = 35），沿着与相应的正常组织。采用免疫组化、定量RT-PCR和Western blot方法检测Ndrg 2和c-Myc.ResultsThe免疫组化分析显示Ndrg 2在甲状腺癌中的表达降低。NDRG 2 mRNA在甲状腺癌组织中的表达水平明显低于癌旁正常组织，而在甲状腺腺瘤组织中的表达水平无明显差异。通过蛋白质印迹法在蛋白质水平上证实了这种差异表达。NDRG 2的表达与性别、年龄、甲状腺癌组织类型及远处转移无关。这一发现为NDRG 2在甲状腺癌发生发展中的重要作用提供了新的见解。未来的研究需要解决NDRG 2的下调是否是从正常甲状腺进展到癌的原因或结果。
BackgroundNDRG2 (N-Myc downstream-regulated gene 2) was initially cloned in our laboratory. Previous results have shown thatNDRG2 expressed differentially in normal and cancer tissues. Specifically,NDRG2 mRNA was down-regulated or undetectable in several human cancers, and over-expression ofNDRG2 inhibited the proliferation of cancer cells.NDRG2 also exerts important functions in cell differentiation and tumor suppression. However, it remains unclear whetherNDRG2 participates in carcinogenesis of the thyroid.MethodsIn this study, we investigated the expression profile of humanNDRG2 in thyroid adenomas and carcinomas, by examining tissues from individuals with thyroid adenomas (n = 40) and carcinomas (n = 35), along with corresponding normal tissues. Immunohistochemistry, quantitative RT-PCR and western blot methods were utilized to determine both the protein and mRNA expression status of Ndrg2 and c-Myc.ResultsThe immunostaining analysis revealed a decrease of Ndrg2 expression in thyroid carcinomas. When comparing adenomas or carcinomas with adjacent normal tissue from the same individual, the mRNA expression level ofNDRG2 was significantly decreased in thyroid carcinoma tissues, while there was little difference in adenoma tissues. This differential expression was confirmed at the protein level by western blotting. However, there were no significant correlations ofNDRG2 expression with gender, age, different histotypes of thyroid cancers or distant metastases.ConclusionOur data indicates thatNDRG2 may participate in thyroid carcinogenesis. This finding provides novel insight into the important role ofNDRG2in the development of thyroid carcinomas. Future studies are needed to address whether the down-regulation ofNDRG2 is a cause or a consequence of the progression from a normal thyroid to a carcinoma.