Hard contact lens-induced metabolic changes in rabbit corneas.
Hard contact lens-induced metabolic changes in rabbit corneas.
复制标题
硬性隐形眼镜引起兔角膜的代谢变化。
DOI：
10.1016/s0014-4835(89)80037-5
复制
发表时间：
1989
影响因子：
3.4
通讯作者：
Cheng,HM
中科院分区：
文献类型：
--
作者：
Tsubota,K;Kenyon,KR;Cheng,HM
角膜接触镜-角膜相互作用的生物化学尚不清楚，尽管先前的研究表明角膜代谢变化可能是形态学改变的潜在因素。使用兔模型，这种相互作用已被检查与31 P核磁共振（NMR）光谱，检测信号主要来自上皮。并辅以电镜和组织化学检查。聚甲基丙烯酸甲酯镜片引起可逆性变化，包括无氧糖酵解的激活和膜代谢产物水平的紊乱。这些变化比长时间闭眼时发生的变化严重得多。细胞退化和膜代谢产物的损失之间似乎存在关联。另一方面，透氧硅橡胶镜片可以维持几乎正常的代谢模式。这些结果表明，角膜对硬性接触透镜配戴的反应是多方面的。
The biochemistry of contact lens-cornea interaction is not well understood, although previous studies have suggested that corneal metabolic changes may be the underlying factor in morphological alterations. Using a rabbit model, this interaction has been examined with 31P nuclear magnetic resonance (NMR) spectroscopy, which detects signals principally from the epithelium. The examination was supplemented with electron microscopy and histochemistry. Polymethylmethacrylate lenses caused reversible changes, including activation of anaerobic glycolysis and disturbance of membrane metabolite levels. These changes were far more severe than those occurring during prolonged eye closure. There appears to be an association between cellular deterioration and loss of membrane metabolites. On the other hand, oxygen-permeable silicone lenses allowed maintenance of nearly normal metabolic patterns. These results show multifaceted corneal response to hard contact lens wear.