Actinomadura montaniterrae sp. nov., isolated from mountain soil.
Actinomadura montaniterrae sp. nov., isolated from mountain soil.
复制标题
DOI：
10.1099/ijsem.0.001196
复制
发表时间：
2016-09
影响因子：
2.8
通讯作者：
Apakorn Songsumanus;T. Kudo;M. Ohkuma;Wongsakorn Phongsopitanun;S. Tanasupawat
中科院分区：
文献类型：
--
作者：
Apakorn Songsumanus;T. Kudo;M. Ohkuma;Wongsakorn Phongsopitanun;S. Tanasupawat
通过多相分类研究，明确了山地土壤放线菌CYP 1 -1BT菌株的分类地位。该菌株在气生菌丝体上产生单个孢子，或偶尔产生孢子链。化学分类学数据支持将CYP 1 -1BT分类为代表马杜拉放线菌属的成员，这是基于肽聚糖中存在内消旋二氨基庚二酸;半乳糖、葡萄糖、马度糖和核糖作为全细胞糖; MK-9（H6）、MK-9（H8）和MK-9（H4）作为主要甲基萘醌; C16：0、10-甲基化C18：0和C18：1ω 9 c作为主要的细胞脂肪酸;和二磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇和磷脂酰肌醇糖苷作为主要的磷脂。DNA G+C含量为74.3mol%。结合形态学和化学分类学特征，将CYP 1 -1BT鉴定为马杜拉放线菌属的一个成员。根据16 S rRNA基因分析结果，CYP 1 - 1 BT与产氮马杜拉放线菌DSM 44137 T密切相关（98.9%）。表型，基因型和DNA-DNA杂交数据支持的假设，CYP 1 -1BT代表一个新的种的属Actinomadura montaniterranisp. nov.的名称提出。模式菌株为CYP 1 -1BT（=JCM 16995 T =KCTC 39784 T =PCU 349 T =TISTR 2400 T）。
The taxonomic position of the mountain soil actinomycete, strain CYP1-1BT, was clarified by a polyphasic study. The strain produced a single spore, or occasionally a chain of spores, on aerial mycelium. Chemotaxonomic data supported the classification of CYP1-1BT as representing a member of the genus Actinomadura on the basis of the presence of meso-diaminopimelic acid in the peptidoglycan; galactose, glucose, madurose and ribose as whole cell sugars; MK-9(H6), MK-9(H8) and MK-9(H4) as dominant menaquinones; C16 : 0, 10-methylated C18 : 0 and C18 : 1ω9c as the major cellular fatty acids; and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol manosides as the predominant phospholipids. The DNA G+C content was 74.3 mol%. On the basis of the combination of morphological and chemotaxonomic characteristics, CYP1-1BT was identified as representing a member of the genus Actinomadura. On the basis of the results of 16S rRNA gene analysis, CYP1-1BT, was shown to be closely related to Actinomadura nitritigenes DSM 44137T (98.9 %). Phenotypic, genotypic and DNA-DNA hybridization data supported the hypothesis that CYP1-1BT represents a novel species of the genus Actinomadura for which the name Actinomadura montaniterrae sp. nov. is proposed. The type strain is CYP1-1BT (=JCM 16995T=KCTC 39784T=PCU 349T=TISTR 2400T).