Generation of a homozygous CRISPR/Cas9-mediated knockout human iPSC line for PTCH1 gene
Generation of a homozygous CRISPR/Cas9-mediated knockout human iPSC line for PTCH1 gene
复制标题
生成 PTCH1 基因纯合 CRISPR/Cas9 介导的敲除人类 iPSC 系
DOI：
10.1016/j.scr.2021.102517
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发表时间：
2021
影响因子：
1.2
通讯作者：
Fu Qihua
中科院分区：
文献类型：
--
作者：
Zu Bailing;Zhang Xiaoqing;You Guoling;Fu Qihua
PTCH1 is the receptor protein of Hedgehog signaling pathway, and Hedgehog pathway plays a vital role in mammalian embryonic development. However, the specific biological role of PTCH1 is incompletely understood for embryonic development. Here, we used a CRISPR/Cas9 genome editing approach to generate a homozygousPTCH1knock-out iPSC line (SCMCi001-A-1) from a healthy donor, which will be a valuable in vitro model to study the pathogenic mechanism of PTCH1 dysfunction in congenital disease.
