A Novel R2R3-MYB Transcription Factor Contributes to Petal Blotch Formation by Regulating Organ-Specific Expression of PsCHS in Tree Peony (Paeonia suffruticosa)
A Novel R2R3-MYB Transcription Factor Contributes to Petal Blotch Formation by Regulating Organ-Specific Expression of PsCHS in Tree Peony (Paeonia suffruticosa)
复制标题
一种新型 R2R3-MYB 转录因子通过调节牡丹 (Paeonia suffruticosa) 器官特异性表达 PsCHS 促进花瓣斑块的形成
DOI：
10.1093/pcp/pcy232
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发表时间：
2019-03-01
影响因子：
4.9
通讯作者：
Wang, Liangsheng
中科院分区：
文献类型：
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作者：
Gu, Zhaoyu;Zhu, Jin;Wang, Liangsheng
花色模式在植物与传粉者的相互作用中起着关键作用，是被子植物进化过程中最常见的适应之一。然而，在非模式生物中，花色图案形成的分子机制还不太清楚。本研究的目的是通过转录组分析和功能实验来鉴定牡丹花瓣斑点形成相关基因。我们确定了一个R2 R3-MYB基因，PsMYB 12，代表一个独特的R2 R3-MYB亚组，与花瓣斑点的发展密切相关的时空表达模式。我们进一步证明，PsMYB 12相互作用的基本螺旋-环-螺旋（bHLH）和WD 40蛋白的调节复合物，直接激活PsCHS的表达，这也是特定的花瓣斑点。总之，这些发现推进了我们对模型植物以外的色素模式形成的分子机制的理解。这些研究成果有利于牡丹花色品种的分子育种和种质创新。
Flower color patterns play critical roles in plant-pollinator interactions and represent one of the most common adaptations during angiosperm evolution. However, the molecular mechanisms underlying flower color pattern formation are less understood in non-model organisms. The aim of this study was to identify genes involved in the formation of petal blotches in tree peony (Paeonia suffruticosa) through transcriptome profiling and functional experiments. We identified an R2R3-MYB gene, PsMYB12, representing a distinct R2R3-MYB subgroup, with a spatiotemporal expression pattern tightly associated with petal blotch development. We further demonstrated that PsMYB12 interacts with a basic helix-loop-helix (bHLH) and a WD40 protein in a regulatory complex that directly activates PsCHS expression, which is also specific to the petal blotches. Together, these findings advance our understanding of the molecular mechanisms of pigment pattern formation beyond model plants. They also benefit molecular breeding of tree peony cultivars with novel color patterns and promote germplasm innovation.