Optical nanosensors - an enabling technology for intracellular measurements
Optical nanosensors - an enabling technology for intracellular measurements
复制标题
DOI：
10.1039/b302174m
复制
发表时间：
2003-01-01
期刊：
影响因子：
4.2
通讯作者：
Aylott, JW
中科院分区：
文献类型：
--
作者：
Aylott, JW
光学纳米传感器已经被设计成利用荧光的灵敏度在细胞内环境中进行定量测量，使用的设备足够小，可以以最小的物理扰动插入活细胞。与广泛使用的基于荧光染料的方法相比，纳米传感器矩阵具有两个关键优势；(1)保护感应成分免受细胞内环境中干扰物种的影响；(2)保护细胞内环境免受感应成分的任何毒性影响。这篇重点文章讨论了纳米传感器技术的最新发展，并研究了更复杂的传感方案的使用，这些方案将扩大可检测和量化分析物的范围。
Optical nanosensors have been designed to utilise the sensitivity of fluorescence for making quantitative measurements in the intracellular environment, using devices that are small enough to be inserted into living cells with a minimum of physical perturbation. Advantages over widely used fluorescence dye based methods are observed because the nanosensor matrix imparts two key benefits; (1) protection of the sensing component from interfering species within the intracellular environment and (2) protection of the intracellular environment from any toxic effects of the sensing component. This Highlight article discusses the recent developments in nanosensor technology and investigates the use of more complex sensing schemes that will expand the range of analytes that can be detected and quantified.