Role of deoxyribonucleic acid polymerase III in the repair of single-strand breaks produced in Escherichia coli deoxyribonucleic acid by gamma radiation
Role of deoxyribonucleic acid polymerase III in the repair of single-strand breaks produced in Escherichia coli deoxyribonucleic acid by gamma radiation
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脱氧核糖核酸聚合酶 III 在修复大肠杆菌脱氧核糖核酸伽马辐射产生的单链断裂中的作用
DOI：
10.1128/jb.127.3.1307-1314.1976
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发表时间：
1976
影响因子：
3.2
通讯作者：
K. Smith
中科院分区：
文献类型：
--
作者：
C. Hamelin;D. A. Youngs;K. Smith
测量 137Cs 伽马射线照射后大肠杆菌 K-12 pol+、polA1、polC1026(ts) 和 polA1 polC1026(ts) 细胞的细胞存活、脱氧核糖核酸 (DNA) 降解和 DNA 单链断裂修复。结果表明 polA+ 或 polA 细胞中生长培养基依赖性（III 型）修复需要 DNA 聚合酶 III。在 pol+ 或 polC 细胞中，DNA 聚合酶 I 有效地执行 II 型修复。这些菌株在 DNA 修复方面的相对缺陷通常与其对细胞杀伤的相对敏感性以及观察到的 DNA 降解程度相关。
Cell survival, deoxyribonucleic acid (DNA) degradation, and the repair of DNA single-strand breaks were measured for Escherichia coli K-12 pol+, polA1, polC1026(ts), and polA1 polC1026(ts) cells after 137Cs gamma irradiation. The results indicate that DNA polymerase III is required for growth medium-dependent (type III) repair in polA+ or polA cells. In pol+ or polC cells, DNA polymerase I performs type II repair efficiently. The relative deficiencies of each of these strains in DNA repair generally correlate with their relative sensitivities to cell killing and with the extent of DNA degradation observed.