A di-acidic signal required for selective export from the endoplasmic reticulum
A di-acidic signal required for selective export from the endoplasmic reticulum
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DOI：
10.1126/science.277.5325.556
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发表时间：
1997-07-25
期刊：
影响因子：
56.9
通讯作者：
Balch, WE
中科院分区：
文献类型：
--
作者：
Nishimura, N;Balch, WE
膜蛋白在细胞内区室之间的转运需要蛋白质胞质结构域中的特定序列来指导包装进入囊泡穿梭。一个序列，介导出口从内质网（ER）已被证明难以捉摸。水泡性口炎病毒糖蛋白（VSV-G）和其他货物分子的胞质尾部上的二酸信号（Asp-X-Glu，其中X代表任何氨基酸）是有效募集到小仓鼠肾细胞中介导从ER输出的囊泡所必需的。这种信号的存在提供了证据，表明从ER的输出是通过选择性机制发生的。
Transport of membrane proteins between intracellular compartments requires specific sequences in the protein cytoplasmic domain to direct packaging into vesicle shuttles. A sequence that mediates export from the endoplasmic reticulum (ER) has proved elusive. A di-acidic signal (Asp-X-Glu, where X represents any amino acid) on the cytoplasmic tail of vesicular stomatitis virus glycoprotein (VSV-G) and other cargo molecules was required for efficient recruitment to vesicles mediating export from the ER in baby hamster kidney cells. The existence of such a signal provides evidence that export from the ER occurs through a selective mechanism.