STERILE HOST YEASTS (SHY) - EUKARYOTIC SYSTEM OF BIOLOGICAL CONTAINMENT FOR RECOMBINANT DNA EXPERIMENTS
STERILE HOST YEASTS (SHY) - EUKARYOTIC SYSTEM OF BIOLOGICAL CONTAINMENT FOR RECOMBINANT DNA EXPERIMENTS
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DOI：
10.1016/0378-1119(79)90004-0
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发表时间：
1979-01-01
期刊：
影响因子：
3.5
通讯作者：
DAVIS, RW
中科院分区：
文献类型：
--
作者：
BOTSTEIN, D;FALCO, SC;DAVIS, RW
描述了一种用于酿酒酵母（酿酒酵母/面包酵母）中重组DNA实验的生物封闭系统。遏制的原则是不育：单倍体宿主菌株都含有交配型非特异性不育突变。宿主还含有4种营养缺陷型突变，适合于选择所用的各种载体。所有载体都是pBR 322的衍生物，可以在酵母和大肠杆菌中选择和维持。该系统最近获得了美国国立卫生研究院HV 2 [宿主载体系统2]水平的认证。
A system of biological containment for recombinant DNA experiments in Saccharomyces cerevisiae (Brewer''s/Baker''s yeast) is described. The principle of containment is sterility: the haploid host strains all contain a mating-type-non-specific sterile mutation. The hosts also contain 4 auxotrophic mutations suitable for selection for the various kinds of vectors used. All vectors are derivatives of pBR322 which can be selected and maintained in both yeast and Escherichia coli. The system was recently certified at the HV2 [host vector system 2] level by the National Institutes of Health.