In situ staining with DNA-binding fluorescent dye, Hoechst 33258, to detect microorganisms in the epithelial cells of oral leukoplakia
In situ staining with DNA-binding fluorescent dye, Hoechst 33258, to detect microorganisms in the epithelial cells of oral leukoplakia
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DOI：
10.1016/s1368-8375(01)00016-1
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发表时间：
2001-09-01
期刊：
影响因子：
4.8
通讯作者：
Mizuki, H
中科院分区：
文献类型：
--
作者：
Mizuki, H
本研究旨在调查白斑上皮细胞中是否存在微生物。将 20 份白斑标本的冰冻切片用 DNA 结合双苯甲亚胺 Hoechst 33258 进行染色。使用 20 份正常口腔粘膜标本和 5 份正常皮肤标本作为对照。在所有白斑标本中，在上皮细胞的细胞质内观察到小颗粒状荧光结构，主要是在棘细胞的细胞质内，尽管颗粒状结构的数量在标本、上皮层甚至单个切片内的上皮区域之间有所不同。在正常粘膜上皮细胞的细胞质中观察到较少的颗粒结构，或者根本没有观察到颗粒结构。在皮肤上皮细胞质中没有观察到结构。这项研究的结果强烈表明微生物存在于口腔粘膜上皮细胞中，并且它们与口腔白斑的发展密切相关。据推测，上皮细胞中的微生物可能是细菌，特别是支原体。 (C) 2001 Elsevier Science Ltd. 保留所有权利。
This study was performed to investigate the presence of microorganisms in the epithelial cells of leukoplakia. Frozen sections of 20 specimens of leukoplakia were stained with DNA-binding bisbenzimide Hoechst 33258. As a control, 20 specimens of normal oral mucosa and five specimens of normal skin were used. In all preparations of leukoplakia, small granular fluorescing structures were observed within the cytoplasm of the epithelial cells, predominantly within the cytoplasm of prickle cells, although the amount of the granular structures varied between specimens, layers of the epithelium and even areas of the epithelium within a single section. Less granular structures were observed, or none at all, in the cytoplasm of the epithelial cells of normal mucosa. No structures were observed in the cytoplasm of the epithelium of skin. The results in this study strongly suggest that microorganisms are present in the epithelial cells of oral mucosa, and that they are closely associated with the development of oral leukoplakia. It is postulated that the microorganisms in the epithelial cells could be bacteria, particularly mycoplasmas. (C) 2001 Elsevier Science Ltd. All rights reserved.