Triacrylamide-Based Adhesives Stabilize Bonds in Physiologic Conditions
Triacrylamide-Based Adhesives Stabilize Bonds in Physiologic Conditions
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DOI：
10.1177/00220345211061736
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发表时间：
2022-01-09
影响因子：
7.6
通讯作者：
Pfeifer，C. S.
中科院分区：
文献类型：
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作者：
de Lucena，F. S.;Lewis，S. H.;Pfeifer，C. S.
在这项研究中，丙烯酰胺基粘合剂与硫代氨基甲酸酯基复合材料相结合，以提高粘结稳定性和降低聚合应力，分别模拟复合材料的双组分。在生理条件下进行稳定性试验，结合机械和细菌挑战。将氨基甲酸酯二甲基丙烯酸酯与新合成的三丙烯酰胺（TMAAEA）或HEMA（2-羟乙基-甲基丙烯酸酯;对照）组合以产生2步全蚀刻粘合剂体系。配制基于甲基丙烯酸酯的复合材料（70重量%硅烷化填料），其含有0（对照）或20重量%的硫代氨基甲酸酯低聚物。恢复人第三磨牙的标准化制剂;然后，从咬合面获得环氧树脂复制品之前和之后7-d存储在水中或与变形链球菌生物膜，这是测试后存储在培养箱（静态）或生物反应器（机械挑战）。从样品的复制品（扫描电子显微镜）和横截面（共聚焦激光扫描显微镜）获得图像，然后进行分析以获得间隙、细菌浸润和脱矿的测量值。微拉伸粘结强度的标本储存在水中或生物膜进行了评估，在1毫米2坚持标本。采用方差分析和Tukey检验（α = 0.05）对数据进行分析。基于HEMA的材料具有更大的初始间隙测量值，表明丙烯酰胺材料的更有效的粘合。当在水中测试时，基于三丙烯酰胺的粘合剂在培养箱或生物反应器中的间隙较小。在生物膜的存在下，材料之间的差异较小，但丙烯酰胺/硫代氨基甲酸酯组合导致生物反应器中统计学上较低的间隙形成。HEMA和TMAAEA为基础的粘合剂产生统计学上相似的微拉伸粘结强度后，在水中储存7天，但在同一时期与生物膜挑战的标本，TMAAEA为基础的粘合剂是唯一的保留初始粘结强度值。使用一种稳定的聚丙烯酰胺基粘合剂导致保存的树脂牙本质粘结界面后，生理相关的挑战。未来的研究将包括多物种生物膜模型。
In this study, an acrylamide-based adhesive was combined with a thiourethane-based composite to improve bond stability and reduce polymerization stress, respectively, of simulated composite restorations. The stability testing was conducted under physiologic conditions, combining mechanical and bacterial challenges. Urethane dimethacrylate was combined with a newly synthesized triacrylamide (TMAAEA) or HEMA (2-hydroxyethyl-methacrylate; control) to produce a 2-step total-etch adhesive system. Methacrylate-based composites (70 wt% silanized filler) were formulated, containing thiourethane oligomers at 0 (control) or 20 wt%. Standardized preparations in human third molars were restored; then, epoxy replicas were obtained from the occlusal surfaces before and after 7-d storage in water or withStreptococcus mutansbiofilm, which was tested after storage in an incubator (static) or the bioreactor (mechanical challenge). Images were obtained from the replicas (scanning electron microscopy) and cross sections of the samples (confocal laser scanning microscopy) and then analyzed to obtain measurements of gap, bacterial infiltration, and demineralization. Microtensile bond strength of specimens stored in water or biofilm was assessed in 1-mm2stick specimens. Data were analyzed with analysis of variance and Tukey’s test (α = 0.05). HEMA-based materials had greater initial gap measurements, indicating more efficient bonding for the acrylamide materials. When tested in water, the triacrylamide-based adhesive had smaller gaps in the incubator or bioreactor. In the presence of biofilm, there was less difference among materials, but the acrylamide/thiourethane combination led to statistically lower gap formation in the bioreactor. HEMA and TMAAEA-based adhesives produced statistically similar microtensile bond strengths after being stored in water for 7 d, but after the same period with biofilm-challenged specimens, the TMAAEA-based adhesives were the only ones to retain the initial bond strength values. The use of a stable multiacrylamide-based adhesive led to the preservation of the resin-dentin bonded interface after a physiologically relevant challenge. Future studies will include a multispecies biofilm model.