Adhesive but not lateral E-cadherin complexes require calcium and catenins for their formation.
Adhesive but not lateral E-cadherin complexes require calcium and catenins for their formation.
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DOI：
10.1083/jcb.142.3.837
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发表时间：
1998-08-10
期刊：
影响因子：
--
通讯作者：
Troyanovsky SM
中科院分区：
文献类型：
--
作者：
Chitaev NA;Troyanovsky SM
我们研究了上皮A-431细胞产生内源性E-钙粘蛋白和重组形式的E-钙粘蛋白标记的myc或旗表位的intercadherin的相互作用。发现了三种不同的E-cadherin复合物：第一种是传统的E-cadherin-catenin复合物，它由一个E-cadherin分子与β-catenin/α-catenin或斑珠蛋白/α-catenin二聚体连接而成。第二种是横向E-钙粘蛋白复合物，其包含通过E-钙粘蛋白的NH 2-末端胞外结构域的二聚化以平行方式组合的两个E-钙粘蛋白-连环蛋白常规复合物。第三种复合物可能含有两个E-钙粘蛋白-连环蛋白传统复合物，它们来自两个相对的细胞，并以反平行的方式排列。形成的反平行，但不是横向复合物严格依赖于细胞外钙和E-钙粘蛋白结合到连环蛋白。双氨基酸取代Trp 156 Ala/Val 157 Gly细胞外NH 2-末端E-钙粘蛋白结构域内完全废除了横向和反平行的E-钙粘蛋白间的关联。这些数据支持了反平行复合物具有粘附功能的观点。此外，他们让我们建议，反平行复合物来自横向二聚体，这个复杂的过程需要连环蛋白和钙离子。
We examined intercadherin interactions in epithelial A-431 cells producing endogenous E-cadherin and recombinant forms of E-cadherin tagged either by myc or by flag epitopes. Three distinct E-cadherin complexes were found. The first is a conventional E-cadherin–catenin complex consisting of one E-cadherin molecule linked either to β-catenin/α-catenin or to plakoglobin/α-catenin dimers. The second is a lateral E-cadherin complex incorporating two E-cadherin– catenin conventional complexes combined in parallel fashion via dimerization of the NH2-terminal extracellular domain of E-cadherin. The third complex is likely to contain two E-cadherin–catenin conventional complexes derived from two opposing cells and arranged in an antiparallel fashion. Formation of the antiparallel but not lateral complex strictly depends on extracellular calcium and E-cadherin binding to catenins. Double amino acid substitution Trp156Ala/Val157Gly within the extracellular NH2-terminal E-cadherin domain completely abolished both lateral and antiparallel inter–E-cadherin association. These data support an idea that the antiparallel complex has the adhesion function. Furthermore, they allow us to suggest that antiparallel complexes derive from lateral dimers and this complex process requires catenins and calcium ions.