lncRNA GHET1 has effects in development of pre-eclampsia
lncRNA GHET1 has effects in development of pre-eclampsia
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lncRNA GHET1对先兆子痫的发展有影响
DOI：
10.1002/jcb.28531
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发表时间：
2019
影响因子：
4
通讯作者：
Zou Yanfen
中科院分区：
文献类型：
--
作者：
Li Shuhong;Liu Jie;Kong Fanqiang;Wang Yuanli;Li Ning;Zou Yanfen
为了解释长链非编码RNA（lncRNA）胃癌高表达转录本1（GHET 1）在先兆子痫发生中的作用机制。通过苏木精-伊红染色评价正常、轻度和重度子痫前期的病理变化，并通过逆转录聚合酶链反应测定不同组织中lncRNA GHET 1的表达。在细胞实验中，将BeWo细胞随机分为正常对照组、模型组和lncRNA组。模型组和lncRNA组的JEG 3细胞在缺氧条件下培养。通过Tanswell和伤口愈合测定来测量JEG 3细胞侵袭和迁移能力。Western blot（WB）检测各组细胞蛋白质相对表达量。与正常产妇相比，子痫前期患者lncRNA GHET 1基因表达显著下调（P< 0.05）。在细胞实验中，模型组的侵袭细胞数和创面愈合率较NC组明显受到抑制（P< 0.05）。而lncRNA GHET 1过表达组的侵袭细胞数和创面愈合率均高于对照组（P< 0.05）。WB法检测E‐cadherin、FN、vimentin蛋白表达，模型组与lncRNA组比较差异有统计学意义（P< 0.05）。lncRNA GHET 1过表达恢复了先兆子痫中缺氧降低的细胞侵袭和迁移能力。
To explain long noncoding RNA (lncRNA) gastric carcinoma high expressed transcript 1 (GHET1) affects the mechanism in development of pre‐eclampsia. The pathological changes of normal, mild, and severe pre‐eclampsia were evaluated by hematoxylin and eosin staining and measured the lncRNA GHET1 expression in different tissues by reverse‐transcription polymerase chain reaction. In the cell experiment, the BeWo cells were randomly divided into three groups: normal control (NC) group, model group, and lncRNA group. The JEG3 cells of the model and lncRNA groups were cultured in the hypoxia condition. The JEG3 cells invasion and migration abilities were measured by Tanswell and wound‐healing assays. The relative protein expressions of different groups were evaluated by Western blot (WB) assay. Compared with normal puerperal, the lncRNA GHET1 gene expression of pre‐eclampsia was significantly downregulated (P< 0.05, respectively). In the cell experiment, the invasion cell number and wound‐healing rate of the model group were significantly suppressed compared with the NC group (P< 0.05, respectively). However, the invasion cell number and wound‐healing rate of lncRNA group were enhanced by lncRNA GHET1 overexpression (P< 0.05, respectively). In WB assay, the E‐cadherin, fibronectin, and vimentin proteins expression showed significant differences between the model and lncRNA groups (P< 0.05, respectively). lncRNA GHET1 overexpression had restored cell invasion and migration abilities reduced by hypoxia in pre‐eclampsia.