Genetic characterization of natural reassortant H4 subtype avian influenza viruses isolated from domestic ducks in Zhejiang province in China from 2013 to 2014
Genetic characterization of natural reassortant H4 subtype avian influenza viruses isolated from domestic ducks in Zhejiang province in China from 2013 to 2014
复制标题
2013-2014年浙江省家鸭自然重配H4亚型禽流感病毒遗传特征
DOI：
10.1007/s11262-015-1245-2
复制
发表时间：
2015-12-01
期刊：
影响因子：
1.6
通讯作者：
Wu, Nanping
中科院分区：
文献类型：
--
作者：
Wu, Haibo;Peng, Xiuming;Wu, Nanping
H4亚型流感病毒于1999年在加拿大首次从患肺炎的猪身上分离出来。 H4 禽流感病毒 (AIV) 能够跨越物种屏障感染人类。为了更好地了解中国东部流行的H4 AIV毒株与亚洲其他AIV毒株之间的遗传关系，2013年至2014年对浙江省活禽市场的家鸭进行了调查。本研究从家鸭中分离出23株H4N2（n = 14）和H4N6（n = 9）毒株，并对这些毒株的所有8个基因片段进行了测序，并与中国现有的参考AIV毒株进行了比较。基因银行。分离的菌株主要聚集在欧亚谱系内。没有观察到与适应哺乳动物宿主或耐药性相关的突变。发现H4重配菌株对小鼠的致病性较低，并且无需事先适应即可在小鼠的肺中复制。鉴于家鸭在导致新 AIV 的重组事件中发挥着重要作用，需要持续监测。
The H4 subtype of the influenza virus was first isolated in 1999 from pigs with pneumonia in Canada. H4 avian influenza viruses (AIVs) are able to cross the species barrier to infect humans. In order to better understand the genetic relationships between H4 AIV strains circulating in Eastern China and other AIV strains from Asia, a survey of domestic ducks in live poultry markets was undertaken in Zhejiang province from 2013 to 2014. In this study, 23 H4N2 (n = 14) and H4N6 (n = 9) strains were isolated from domestic ducks, and all eight gene segments of these strains were sequenced and compared to reference AIV strains available in GenBank. The isolated strains clustered primarily within the Eurasian lineage. No mutations associated with adaption to mammalian hosts or drug resistance was observed. The H4 reassortant strains were found to be of low pathogenicity in mice and able to replicate in the lung of the mice without prior adaptation. Continued surveillance is required, given the important role of domestic ducks in reassortment events leading to new AIVs.