Nanofibrous membranes containing reactive groups:: Electrospinning from poly(acrylonitrile-co-maleic acid) for lipase immobilization
Nanofibrous membranes containing reactive groups:: Electrospinning from poly(acrylonitrile-co-maleic acid) for lipase immobilization
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DOI：
10.1021/ma0517998
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发表时间：
2006-02-07
期刊：
影响因子：
5.5
通讯作者：
Seta, P
中科院分区：
文献类型：
--
作者：
Ye, P;Xu, ZK;Seta, P
以聚丙烯腈-共马来酸（PANCMA）为原料，采用静电纺丝法制备了新型活性羧基纳米纤维膜。用场发射扫描电镜对其形貌和纤维直径进行了分析。通过改变溶液浓度，纤维直径可在100 ~ 600 nm范围内变化。在1-乙基-3（（二甲氨基）丙基）盐酸碳二亚胺/ n -羟基琥珀酰亚胺的存在下，通过羧基活化将假丝酵母脂肪酶共价固定在膜表面。测定了固定化脂肪酶在纳米纤维和中空纤维PANCMA膜上的性能。结果表明，与中空纤维膜相比，固定化脂肪酶在纳米纤维膜上的酶载量和活性保持率分别从2.36 +/- 0.06和33.9提高到21.2 +/- 0.7 mg/g和37.6%。测定了游离脂肪酶和固定化脂肪酶的动力学常数K-m和V-max。结果表明，两种固定化酶的V-max值相似，但固定化酶的K-m值从中空纤维膜上的1.36降至纳米纤维膜上的0.98。所研究的脂肪酶固定化纳米纤维可作为生物催化剂用于聚酯合成和/或原位形成纳米纤维增强复合材料。
Novel nanofibrous membranes containing reactive carboxyl groups were fabricated from poly(acrylonitrile-co-maleic acid) (PANCMA) by the electrospinning process. The morphology and fiber diameter were analyzed with field emission scanning electron microscopy. It was found that the fiber diameter could be varied from 100 to 600 nm by changing the solution concentration. Lipase from Candida rugosa was covalently immobilized onto the membrane surface via the activation of carboxyl groups in the presence of 1-ethyl-3((dimethylamino)propyl)carbodiimide hydrochloride/N-hydroxylsuccinimide. The properties of the immobilized lipases on the nanofibrous and hollow fiber PANCMA membranes were measured. It was found that, compared with the hollow fiber membrane, the enzyme loading and the activity retention of the immobilized lipase on the nanofibrous membrane increase from 2.36 +/- 0.06 to 21.2 +/- 0.7 mg/g and from 33.9 to 37.6%, respectively. The kinetic constants of the free and immobilized lipases, K-m and V-max, were assayed. Results indicate that the V-max values are similar for both immobilized enzymes, while the K-m value of the immobilized enzyme decreases from 1.36 on the hollow fiber membrane to 0.98 on the nanofibrous membrane. The studied lipase-immobilized nanofibers can be used as biocatalysts for polyester synthesis and/or in situ formation of nanofiber reinforcement composites.