Paternal methyl donor deficient diets during development affect male offspring behavior and memory-related gene expression in mice.
Paternal methyl donor deficient diets during development affect male offspring behavior and memory-related gene expression in mice.
复制标题
发育过程中父亲甲基供体缺乏的饮食会影响小鼠雄性后代的行为和记忆相关基因的表达。
DOI：
10.1002/dev.21801
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发表时间：
2019
影响因子：
2.2
通讯作者：
Shimizu E.
中科院分区：
文献类型：
--
作者：
Sahara Y;Matsuzawa D;Ishii D;Fuchida T;Goto T;Sutoh C;Shimizu E.
越来越明显的是，被称为表观遗传标记的DNA甲基化会影响动物的行为。在我们之前的研究中，青少年时期的甲基供体（叶酸、蛋氨酸和胆碱）缺乏（FMCD）饮食可能会影响焦虑样行为和恐惧记忆，并伴有海马中某些基因表达及其甲基化的改变。一个问题是，父母的恐惧记忆是否会影响后代的恐惧反应。为了探讨这一问题，本研究在3 ~ 12周龄时，给C57BL/6 J雄性（F0）小鼠喂食FMCD饮食。在确认FMCD饮食对F0小鼠行为和基因表达的影响后，使用相同的行为电池和基因分析检查了它们的雄性后代（F1‐FMCD小鼠）。我们观察到基于F0饮食的F1行为差异，以及海马中记忆相关基因（Camk2α和PP1）表达和PP1基因启动子甲基化的差异。我们的研究结果增加了证据，表明F1代的行为和基因表达可能由于父亲甲基供体营养素摄入量的差异而改变。
It has become increasingly evident that the methylation of DNA, known as an epigenetic marker, affects behavior in animals. In our previous study, a methyl‐donors (folate, methionine, and choline)‐deficient (FMCD) diet during the juvenile period could be shown to affect anxiety‐like behavior and fear memory, accompanied by alteration in some gene expression and their methylations in the hippocampus. One question is whether the fear memory of a parent affects the fear responses of offspring. To explore this question in the present study, C57BL/6 J male (F0) mice were given a FMCD diet from 3 to 12 weeks of age. After confirming the effect of the FMCD diet on the behavior and gene expression of F0 mice, their male offspring (F1‐FMCD mice) were examined using the same behavioral batteries and genetic analysis. F0 diet‐based differences in F1 behavior were observed, accompanied by the differences in the expression of memory‐related genes (Camk2α and PP1) and promoter methylation of the PP1 gene in the hippocampus. Our results add evidence that behavior and gene expression of the F1 generation could be altered due to differences in the father's intake of methyl‐donor nutrients.