Evaluation of AFLP, A High‐Resolution DNA Fingerprinting Method, as a Tool for Molecular Subtyping of Enterohemorrhagic Escherichia coli O157:H7 Isolates
Evaluation of AFLP, A High‐Resolution DNA Fingerprinting Method, as a Tool for Molecular Subtyping of Enterohemorrhagic Escherichia coli O157:H7 Isolates
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AFLP（一种高分辨率 DNA 指纹图谱方法）作为肠出血性大肠杆菌 O157:H7 分离株分子分型工具的评估
DOI：
10.1111/j.1348-0421.1999.tb02473.x
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发表时间：
1999
影响因子：
2.6
通讯作者：
Haruo Watanabe
中科院分区：
文献类型：
--
作者：
Sunao Lyoda;A. Wada;Jeniffer Weller;S. Flood;E. Schreiber;Bill Tucker;Haruo Watanabe
扩增片段长度多态性 (AFLPTM) 技术基于限制性片段的选择性 PCR 扩增。我们研究了 AFLP 在肠出血性大肠杆菌 O157:H7 血清型分离株分子分型中的应用。我们分析了总共 46 个大肠杆菌 O157:H7 分离株以及其他血清型 O26:H11、O114:H19 和 O119:NT。来自同一爆发的大肠杆菌 O157:H7 分离株显示出相同的 AFLP 带模式，并被分型到同一组中，给出的结果几乎与脉冲场凝胶电泳 (PFGE) 研究的结果一致，而其他血清型则显示出与大肠杆菌 O157:H7 明显不同的模式。这些结果表明 AFLP 技术有潜力作为大肠杆菌 O157:H7 分子流行病学的替代工具。
The amplified fragment‐length polymorphism (AFLPTM) technique is based on the selective PCR amplification of restriction fragments. We investigated the utility of AFLP in the molecular subtyping of enterohemorrhagic Escherichia coli serotype O157:H7 isolates. We analyzed a total of 46 isolates of E. coli O157:H7 along with other serotypes, O26:H11, O114:H19 and O119:NT. Isolates of E. coli O157:H7 derived from the same outbreak showed an identical AFLP‐banding pattern and were subtyped into the same group, giving results almost consistent with those of a pulsed‐field gel electrophoresis (PFGE) study, while other serotypes showed clearly different patterns from those of E. coli O157:H7. These results suggest that the AFLP technique has potential as an alternative tool for the molecular epidemiology of E. coli O157:H7.