Chemical assistance in refolding of bacterial inclusion bodies.
Chemical assistance in refolding of bacterial inclusion bodies.
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DOI：
10.1155/2011/631607
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发表时间：
2011
影响因子：
3
通讯作者：
Mirzahoseini H
中科院分区：
文献类型：
--
作者：
Alibolandi M;Mirzahoseini H
大肠杆菌是重组蛋白生产中应用最广泛的宿主之一，但外源蛋白的难溶表达是大肠杆菌生产重组蛋白的主要瓶颈。在体外将包涵体重折叠成具有天然构象的蛋白质是解决这一问题的一种方法，但需要对每种蛋白质的特定条件进行优化。已经描述了几种体外复性的方法，其中大多数涉及使用添加剂来辅助正确的折叠。共溶物在复性过程中起主要作用，根据其作为聚集抑制物和折叠促进剂的功能可分为两类。本文综述了不溶性重组蛋白小规模复性过程中使用的添加剂和工业化过程中使用的添加剂。
Escherichia coli is one of the most widely used hosts for the production of recombinant proteins but insoluble expression of heterologous proteins is a major bottleneck in production of recombinant proteins in E. coli. In vitro refolding of inclusion body into proteins with native conformations is a solution for this problem but there is a need for optimization of condition for each protein specifically. Several approaches have been described for in vitro refolding; most of them involve the use of additives for assisting correct folding. Cosolutes play a major role in refolding process and can be classified according to their function as aggregation suppressors and folding enhancers. This paper presents a review of additives that are used in refolding process of insoluble recombinant proteins in small scale and industrial processes.