Transcriptional profiling of circadian patterns of mRNA expression in the chick retina
Transcriptional profiling of circadian patterns of mRNA expression in the chick retina
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DOI：
10.1074/jbc.m405679200
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发表时间：
2004-12-10
影响因子：
4.8
通讯作者：
Cassone, VM
中科院分区：
文献类型：
--
作者：
Bailey, MJ;Beremand, PD;Cassone, VM
先前的转录组分析已经鉴定了鸟类松果体时钟的候选分子成分，本文中我们采用松果体转录物的高密度 cDNA 微阵列来确定每日和持续黑暗条件下小鸡视网膜中的振荡转录物。随后对松果体和视网膜振荡器的比较转录组分析区分了两者之间的一些转录相似性以及显着差异。节律性视网膜转录本根据功能类别进行分类，包括光转导元件、转录/翻译因子、载体蛋白、细胞信号分子和应激反应基因。候选视网膜时钟转录本也根据一天中不同时间的 mRNA 丰度进行组织，揭示了各组在整个昼夜节律中积累峰值 mRNA 水平，但主要在主观黎明或主观黄昏时达到峰值。在恒定条件下，将小鸡视网膜转录组与松果体转录组进行比较，得出一组有趣的保守基因。该组包括假定的时钟元件cry1和per3，以及几个先前未识别和未研究的基因，这些基因表现出在日常和恒定条件下显着变化的mRNA丰度特征。相比之下，许多转录本受到差异性调节，包括那些被认为参与褪黑激素生物合成和生物钟机制的转录本。我们的结果表明鸟类松果体和视网膜之间存在密切的转录关系，此外还提供了我们假设参与昼夜节律产生的先前未表征的分子元件。
Previous transcriptome analyses have identified candidate molecular components of the avian pineal clock, and herein we employ high density cDNA microarrays of pineal gland transcripts to determine oscillating transcripts in the chick retina under daily and constant darkness conditions. Subsequent comparative transcriptome analysis of the pineal and retinal oscillators distinguished several transcriptional similarities between the two as well as significant differences. Rhythmic retinal transcripts were classified according to functional categories including phototransductive elements, transcription/translation factors, carrier proteins, cell signaling molecules, and stress response genes. Candidate retinal clock transcripts were also organized relative to time of day mRNA abundance, revealing groups accumulating peak mRNA levels across the circadian day but primarily reaching peak values at subjective dawn or subjective dusk.Comparison of the chick retina transcriptome to the pineal transcriptome under constant conditions yields an interesting group of conserved genes. This group includes putative clock elements cry1 and per3 in addition to several previously unidentified and uninvestigated genes exhibiting profiles of mRNA abundance that varied markedly under daily and constant conditions. In contrast, many transcripts were differentially regulated, including those believed to be involved in both melatonin biosynthesis and circadian clock mechanisms. Our results indicate an intimate transcriptional relationship between the avian pineal and retina in addition to providing previously uncharacterized molecular elements that we hypothesize to be involved in circadian rhythm generation.