Spirochetes from digital dermatitis lesions in cattle are closely related to treponemes associated with human periodontitis
Spirochetes from digital dermatitis lesions in cattle are closely related to treponemes associated with human periodontitis
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DOI：
10.1099/00207713-47-1-175
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发表时间：
1997-01-01
期刊：
影响因子：
--
通讯作者：
Gobel, UB
中科院分区：
文献类型：
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作者：
Choi, BK;Nattermann, H;Gobel, UB
指皮炎（DD），首先由Cheli和Mortellaro（R. Cheli和C. Mortellaro，p.208 -213，在Proceedings of the 8 th International Conference on Diseases of Cattle，1974中）是奶牛和肉牛中的主要问题，在全世界造成重大经济损失。病变通常发现于足跟球近端的掌侧皮肤，这些病变的活检或触摸制备的显微镜检查揭示了各种不同的细菌形态型，包括大量的螺旋体，其通常代表主要的形态型。结果表明，迄今为止所观察的这些病变至少包含5种螺旋体的亚型，它们都聚在密螺旋体属内，其中DDKL-4型与非致病性人类密螺旋体T的亲缘关系近（99.4%）。噬菌体DDKL-3和DDKL-13两种密螺旋体类型与人类牙周炎病变中常见的密螺旋体密切相关，即，T. denticola和T. vincentii，分别表现出95%和98%的相似性。另外两种密螺旋体类型，DDKL-12和DDKL-20，与任何可培养密螺旋体物种没有近亲，但与先前描述的IV组口腔密螺旋体聚类。用荧光标记的寡核苷酸探针对DD活检涂片进行原位杂交的初步分析表明，在迄今为止分析的所有病变中，T。齿垢样螺旋体在所有螺旋体形态型中的比例最高。
Digital dermatitis (DD), first described in 1974 by Cheli and Mortellaro (R. Cheli and C. Mortellaro, p. 208-213, in Proceedings of the 8th International Conference on Diseases of Cattle, 1974), is a major problem in dairy cows and beef cattle causing significant economic losses worldwide. Lesions are typically found at the volar skin proximal to the heel bulbs, Microscopic examination of biopsies or touch preparations of these lesions revealed a variety of different bacterial morphotypes including significant numbers of spirochetes which often represent the predominant morphotype. We used comparative 16S rRNA sequence analysis to determine the diversity and phylogeny of these hitherto unclassified DD spirochetes, Results indicate that those lesions looked at so far contained at least five spirochetal phylotypes, all clustering within the genus Treponema, Phylotype DDKL-4 was nearly identical (99.4% similarity) to that of a nonpathogenic human treponeme, T. phagedenis. Two phylotypes DDKL-3 and DDKL-13 were closely related to those from treponemes commonly found in human periodontitis lesions, i.e., T. denticola and T. vincentii, exhibiting 95 and 98% similarity, respectively. The other two phylotypes, DDKL-12 and DDKL-20, had no close relatives to any cultivable treponemal species but clustered to previously described group IV oral treponemes. Preliminary analysis using in situ hybridization with fluorescently labeled oligonucleotide probes against smears from DD biopsies revealed that from all lesions analyzed so far, T. denticola like spirochetes were detected in the highest proportion of all spirochetal morphotypes.