The expression pattern of alfalfa flavanone 3-hydroxylase promoter-gus fusion in Nicotiana benthamianna correlates with the presence of flavonoids detected in situ
The expression pattern of alfalfa flavanone 3-hydroxylase promoter-gus fusion in Nicotiana benthamianna correlates with the presence of flavonoids detected in situ
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DOI：
10.1007/bf00019549
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发表时间：
1996-03-01
影响因子：
5.1
通讯作者：
Ratet, P
中科院分区：
文献类型：
--
作者：
Charrier, B;Leroux, C;Ratet, P
黄烷酮3-羟化酶是在类黄酮生物合成途径的中心部分起作用的酶。它通常由单个基因编码，并且似乎在该途径中具有调控的关键位置。这两个特征使得单一的f3 h启动子-gus融合体成为研究f3 h表达和该途径调控的合适工具。我们在这里提出的表达的苜蓿黄烷酮3-羟化酶（f3 h）启动子gus融合到本塞姆氏烟草的空间和时间分析。苜蓿f3 h启动子指导gus在花、茎、叶和根中表达。在花中，GUS活性在花粉粒、胚珠、子房胎座和表皮、髓薄壁组织、毛状体和围绕花序梗维管束的第二皮层细胞层中观察到。在茎中，GUS活性检测在相同的地方，在花序梗除了髓薄壁组织。在根中，我们发现GUS染色在根毛、表皮和伸长区的维管束中。最后，在叶片中，f3 h启动子基本上在多细胞毛状体的柄细胞中表达。f3 h-gus融合蛋白的表达模式与原位类黄酮的存在相关。这些数据表明，该构建体可以是非常有用的，以研究控制黄酮类化合物的生产的因素。
Flavanone 3-hydroxylase is an enzyme acting in the central part of the flavonoid biosynthesis pathway. It is generally encoded by a single gene and seems to have a key position for the regulation in this pathway. These two features make a single f3h promoter-gus fusion a suitable tool to study both the f3h expression and the regulation of this pathway. We present here the spatial and temporal analysis of the expression of an alfalfa flavanone 3-hydroxylase (f3h) promoter-gus fusion introduced into Nicotiana benthamiana. The Medicago sativa (alfalfa) f3h promoter directed gus expression in flowers, stems, leaves and roots. In flowers, GUS activity was observed in pollen grains, in ovules, in ovary placenta and in the epidermis, medullary parenchyma, trichomes and second cortical cellular layer surrounding the vascular bundles of the peduncle. In stems, GUS activity was detected at the same places as in the peduncle except for the medullary parenchyma. In roots, we found GUS staining in root hairs, epidermis and in the vascular bundles of the elongated zone. Finally, in leaves, the f3h promoter expressed essentially in the stalk cells of the multicellular trichomes. The expression pattern of the f3h-gus fusion was correlated to the presence of flavonoids in situ. These data indicate that this construct can be very useful to study factors controlling the production of flavonoids.