Temperature dependence of intracellular pH in higher plant cells

Temperature dependence of intracellular pH in higher plant cells
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高等植物细胞内 pH 值的温度依赖性

DOI:
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发表时间:
2004
期刊:
影响因子:
4.3
通讯作者:
F. Podo
F. Podo
中科院分区:
生物学2区
文献类型:
--
作者:
P. Aducci;R. Federico;G. Carpinelli;F. Podo

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最近引进的31 P核磁共振光谱法为同时直接评价高等植物细胞的细胞质和液泡pH值提供了一种新的方法(J.K. M. Roberts,P.M. Ray,N. Waderlardetzky和O. Sardetzky,1980,Nature 283,870-872; 1981,Planta 152,74-78)。使用这种方法,我们已经能够检测到当温度从4 ° C升高到28°C时,玉米根尖细胞质区室水平上的选择性pH降低约0.5个单位。这种效应是完全可逆的温度。在液泡区室的水平上没有检测到pH值的变化。
The recent introduction of 31P nuclear magnetic resonance spectroscopy offers a new approach to the problem of obtaining a simultaneous and direct evaluation of both the cytoplasmic and vacuolar pH in higher plant cells (J. K. M. Roberts, P.M. Ray, N. Waderlardetzky and O. Sardetzky, 1980, Nature 283, 870–872; 1981, Planta 152, 74–78). Using this method we have been able to detect a selective pH decrease of about 0.5 units at the level of the cytoplasmic compartment of maize root tips when the temperature was increased from 4 to 28°C. This effect was completely reversible with temperature. No pH variation could be detected at the level of the vacuolar compartment.
DOI: 10.1021/bi00522a006
发表时间: 1981-09
期刊: Biochemistry
影响因子: 2.9
作者:
Justin K. M. Roberts;N. Wade-Jardetzky;O. Jardetsky
通讯作者: Justin K. M. Roberts;N. Wade-Jardetzky;O. Jardetsky