Tributyltin-binding protein type 1, a lipocalin, prevents inhibition of osteoblastic activity by tributyltin in fish scales
Tributyltin-binding protein type 1, a lipocalin, prevents inhibition of osteoblastic activity by tributyltin in fish scales
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三丁基锡结合蛋白 1 型（一种脂质运载蛋白）可防止鱼鳞中三丁基锡对成骨细胞活性的抑制
DOI：
10.1016/j.aquatox.2011.02.009
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Y.
中科院分区：
文献类型：
--
作者：
Satone;H.;Lee;J.M.;Oba;Y.;Kusakabe;T.;Akahoshi;E.;Miki;S.;Suzuki;N.;Sasayama;Y.;Nassef;M.;Shimasaki;Y.;Kawabata;S.;Honjo;T.;Oshima;Y.
三丁基锡结合蛋白1型（TBT-bp 1）是脂质运载蛋白家族的一员，与小的疏水分子结合。在本研究中，我们表达了重组TBT-bp 1（rTBT-bp 1，ca. 35 kDa），并从注射重组杆状病毒的家蚕幼虫血淋巴中纯化了该蛋白。在孵育rTBT-bp 1和TBT的混合物并通过凝胶过滤色谱对其进行分级分离后，在rTBT-bp 1的洗脱峰中检测到TBT，证实了rTBT-bp 1对TBT的结合潜力。rTBT-bp 1或天然TBT-bp 1（nTBT-bp 1）恢复TBT抑制的成骨细胞活性的能力的测定表明，与rTBT-bp 1或nTBT-bp 1结合TBT的鳞片共同治疗恢复了金鱼鳞片中的成骨细胞活性，而单独使用TBT的治疗显著抑制了成骨细胞活性。这些结果表明，TBT-bp 1作为脂质运载蛋白成员可能通过与TBT结合来降低TBT的毒性。
Tributyltin-binding protein type 1 (TBT-bp1) is a member of the lipocalin family of proteins which bind to small hydrophobic molecules. In this study, we expressed a recombinant TBT-bp1 (rTBT-bp1, ca. 35 kDa) in a baculovirus expression system and purified the protein from the hemolymph of silkworm larvae injected with recombinant baculovirus. After incubation of a mixture of rTBT-bp1 and TBT and its fractionation by means of gel filtration chromatography, TBT was detected in the elution peak of rTBT-bp1, confirming the binding potential of rTBT-bp1 for TBT. An assay of the ability of rTBT-bp1 or native TBT-bp1 (nTBT-bp1) to restore osteoblastic activity inhibited by TBT showed that co-treatment of the scales with rTBT-bp1 or nTBT-bp1 in combination with TBT restored osteoblastic activity in goldfish scales, whereas treatment with TBT alone significantly inhibited osteoblastic activity. These results suggest that TBT-bp1 as a lipocalin member might function to decrease the toxicity of TBT by binding to TBT.