An integrated high density RFLP-AFLP map of tomato based on two Lycopersicon esculentum x L-pennellii F2 populations
An integrated high density RFLP-AFLP map of tomato based on two Lycopersicon esculentum x L-pennellii F2 populations
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DOI：
10.1007/s001220051231
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发表时间：
1999-07-01
影响因子：
5.4
通讯作者：
Peleman, J
中科院分区：
文献类型：
--
作者：
Haanstra, JPW;Wye, C;Peleman, J
两个独立的F-2群体的番茄× L。pennellii的RFLP作图研究，用于构建一个高度饱和的整合AFLP图谱。该图谱全长1482 cM，包含67个RFLP标记，其中22对EcoRI + MseI引物组合获得1078个AFLP标记，5对PstI + MseI引物组合获得97个AFLP标记，其中231个AFLP标记为两种群共有标记。EcoRI + MseI AFLP标记在染色体上分布不均匀。在着丝粒区，848个EcoRI + MseI AFLP标记聚在一起，遗传距离为199 cM，相当于每0.23 cM有1个EcoRI + MseI AFLP标记;在远心区，1283 cM被230个EcoRI + MseI AFLP标记聚在一起，相当于每5.6 cM有1个标记。Pst I/MseI AFLP标记分布较均匀，其中16个Pst I/MseI AFLP标记覆盖了199 cM的遗传距离，81个Pst I/MseI AFLP标记覆盖了1283 cM的遗传距离，分别相当于每12和16 cM有1个标记。在这两个群体中，大量基因座表现出显着的偏态分离，但只有第10号染色体基因座表现出两个群体相似的偏态。这种超密集的分子标记图谱为遗传和育种目的以及基于图位的克隆提供了良好的前景。
Two independent F-2 populations of Lycopersicon esculentum x L. pennellii which have previously been investigated in RFLP mapping studies were used for construction of a highly saturated integrated AFLP map. This map spanned 1482 cM and contained 67 RFLP markers, 1078 AFLP markers obtained with 22 EcoRI + MseI primer combinations and 97 AFLP markers obtained with five PstI + MseI primer combinations, 231 AFLP markers being common to both populations. The EcoRI + MseI AFLP markers were not evenly distributed over the chromosomes. Around the centromeric region, 848 EcoRI + MseI AFLP markers were clustered and covered a genetic distance of 199 cM, corresponding to one EcoRI + MseI AFLP marker per 0.23 cM; on the distal parts 1283 cM, were covered by 230 EcoRI + MseI AFLP markers, corresponding to one marker per 5.6 cM. The PstI/MseI AFLP markers showed a more even distribution with 16 PstI/MseI AFLP markers covering a genetic distance of 199 cM around the centromeric regions and 81 PstI/MseI AFLP markers covering a genetic distance of 1283 cM on the more distal parts, corresponding to one marker per 12 and 16 cM respectively. In both populations a large number of loci showed a significant skewed segregation, but only chromosome 10 loci showed skewness that was similar for both populations. This ultra-dense molecular-marker map provides good perspectives for genetic and breeding purposes and map-based cloning.