Expression of bcl2 family proteins and active caspase 3 in classical Hodgkin's lymphomas
Expression of bcl2 family proteins and active caspase 3 in classical Hodgkin's lymphomas
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DOI：
10.1016/j.humpath.2006.06.017
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发表时间：
2007-01-01
期刊：
影响因子：
3.3
通讯作者：
Kanavaros, Panagiotis
中科院分区：
文献类型：
--
作者：
Bai, Maria;Papoudou-Bai, Alexandra;Kanavaros, Panagiotis
各种bcl 2家族蛋白的表达已被报道在霍奇金和Reed-Sternberg细胞中，但蛋白bad、bid和bim尚未在经典霍奇金淋巴瘤（HL）中分析。本研究旨在研究bcl 2、bcl-xl、mcl 1、bax、巴克、bad、bid、bim和活性caspase 3蛋白的表达，以及TUNEL（末端脱氧核苷酸转移酶介导的原位标记）指数，以进一步了解经典HL的凋亡特征。在HRS细胞中发现27/101（27%）、95/101（94%）、27/97（29%）、73/95（77%）、37/102（36%）、85/94（90%）、109例中19例（17%），91例中43例（47%）。bcl-xl、bax和bad在大多数经典HL的HRS细胞中的高表达表明这些蛋白可能在经典HL的凋亡调节中起主导作用。在70例中的47例（67%;范围，0%-12%）和71例中的60例（85%;范围，0%-19%）中分别检测到活性caspase 3阳性和TUNEL阳性Reed-Sternberg细胞。bax/bcl 2（P=.002）、bad/bcl 2（P =.020）、bad/bcl xl（P=.003）和biro/mcl 1（P=.036）之间存在显著正相关。基于这些发现，可以假设抗凋亡蛋白bcl 2、bcl-xl和incl 1可以抵消促凋亡蛋白bax、bad和bim的表达，从而有助于Reed-Sternberg细胞的存活。(c)2007年爱思唯尔公司All rights reserved.
The expression of various bcl2 family proteins has been reported in Hodgkin and Reed-Sternberg cells, but the proteins bad, bid, and bim have not been analyzed in classical Hodgkin's lymphomas (HLs). This study aimed to investigate the expression of the proteins bcl2, bcl-xl, mcl1, bax, bak, bad, bid, bim, and active caspasc 3, and the TUNEL (terminal deoxynucleotidyl transferasemediated in situ labeling) index to gain further insight into the apoptosis profile of classical HLs. A high expression of the proteins bcl2, bcl-xl, mcl1, bax, bak, bad, bid, and bim in HRS cells was found in 27 of 10 1 (27%), 95 of 101 (94%), 27 of 97 (29`%), 73 of 95 (77%), 37 of 102 (36%), 85 of 94 (90%), 19 of 109 (17%), and 43 of 91 (47%) cases, respectively. The high expression of bcl-xl, bax, and bad in HRS cells in most classical HLs indicates that these proteins may play predominant roles in the regulation of apoptosis in classical HLs. Active caspase 3-positive and TUNEL-positive Reed-Sternberg cells were detected in 47 of 70 (67%; range, 0%-12%) and 60 of 71 (85%; range, 0%-19%) cases, respectively. Significant positive correlations were found between bax/bcl2 (P=.002), bad/bcl2 (P=.020), bad/bclxl (P=.003), and biro/mcl1 (P=.036). Based on these findings, it could be hypothesized that the antiapoptotic proteins bcl2, bcl-xl, and incl l may counteract the expression of the proapoptotic proteins bax, bad, and bim, thereby contributing to the survival of Reed-Sternberg cells. (c) 2007 Elsevier Inc. All rights reserved.