Modulation of retinal wound healing by systemically administered bone marrow-derived mesenchymal stem cells.
Modulation of retinal wound healing by systemically administered bone marrow-derived mesenchymal stem cells.
复制标题
通过系统施用的骨髓衍生的间充质干细胞对视网膜伤口愈合的调节。
DOI：
10.3341/kjo.2011.25.4.268
复制
发表时间：
2011-08
影响因子：
--
通讯作者：
Hong DS
中科院分区：
文献类型：
--
作者：
Chung JK;Park TK;Ohn YH;Park SK;Hong DS
评价全身注射骨髓间充质干细胞（MSCs）是否可以整合到视网膜神经组织中，并在激光诱导的视网膜创伤模型中发挥重要作用。通过应用Nd：YAG激光对大鼠视网膜进行视网膜切开术。在损伤后的第一天，在实验组中通过尾静脉注射从同一系大鼠获得的细胞悬液（含有1 × 106个绿色荧光蛋白[GFP]标记的骨髓来源的MSC），并且在对照组中以相同的方式注射磷酸盐缓冲溶液（PBS）。连续拍摄眼底照片进行眼底检查，摘除眼球进行组织学研究，这些研究在MSC和PBS注射后5周和7周进行。制备组织后，常规组织学染色和共聚焦显微镜观察视网膜切开部位。实验组视网膜脱离消退，而对照组视网膜脱离进展。在MSC注射后5周，视网膜切开部位部分闭合，具有可识别的GFP阳性细胞。注射MSC后7周，视网膜完全愈合，无视网膜脱离，在损伤与正常视网膜之间的过渡区观察到大量GFP阳性细胞。全身给药的GFP标记的MSC可以并入神经视网膜组织中，并在物理损伤的视网膜组织的创伤调节中发挥重要作用。
To evaluate whether systemically injected bone marrow-derived mesenchymal stem cells (MSCs) can be incorporated into neuroretinal tissues and play an important role in retinal wound healing in the laser-induced retinal trauma model. Retinotomies were made by applying an Nd:YAG laser to rat retina. On the first day after the injuries, cell suspensions that were obtained from the same line of rat (containing 1 × 106 green fluorescence protein [GFP]-marked bone marrow-derived MSCs) were injected through a tail vein in the experimental group and phosphate buffer solution (PBS) was injected in the same way in the control group. Fundus photographs were taken serially for fundus examination and eyeballs were enucleated for histological studies that were conducted at five and seven weeks after MSC and PBS injection. After the tissues were prepared, the retinotomy sites were observed with routine histological staining and confocal microscopy. Retinal detachment resolved in the experimental group, whereas it progressed in the control group. The retinotomy sites closed partially with identifiable GFP positive cells 5 weeks after MSC injection. At 7 weeks after MSC injection, complete healing without retinal detachment and plentiful GFP positive cells were observed at the transitional zone between damaged and normal retina. Systemically administered GFP-marked MSCs may be incorporated into the neuroretinal tissues and play an important role in the wound modulation of physically damaged retinal tissues.