Rhamnose-binding Lectins from Steelhead Trout (Oncorhynchus mykiss) Eggs Recognize Bacterial Lipopolysaccharides and Lipoteichoic Acid
Rhamnose-binding Lectins from Steelhead Trout (Oncorhynchus mykiss) Eggs Recognize Bacterial Lipopolysaccharides and Lipoteichoic Acid
复制标题
DOI：
10.1271/bbb.66.604
复制
发表时间：
2002-01
期刊：
影响因子：
--
通讯作者：
H. Tateno;T. Ogawa;K. Muramoto;H. Kamiya;M. Saneyoshi
中科院分区：
文献类型：
--
作者：
H. Tateno;T. Ogawa;K. Muramoto;H. Kamiya;M. Saneyoshi
研究了虹鳟（Oncorhynchus mykiss）卵中3种L-鼠李糖结合凝集素（STL 1、STL 2和STL 3）与细菌的相互作用。虽然STL绑定到大多数革兰氏阴性和革兰氏阳性细菌，他们凝集大肠杆菌K-12和枯草芽孢杆菌中的细菌测试。结合被L-鼠李糖抑制。STLs与不同血清型的脂多糖（LPS）结合，并且与大肠杆菌K-12和福氏志贺菌1A的光滑型LPS的结合活性远高于其相应的粗糙型LPS。STL还结合到枯草芽孢杆菌的脂磷壁酸（LTA）。这些结果表明，STL通过识别细胞表面上的LPS或LTA与细菌结合。
The interaction between bacteria and three L-rhamnose-binding lectins, named STL1, STL2, and STL3, from steelhead trout (Oncorhynchus mykiss) eggs was investigated. Although STLs bound to most Gram-negative and Gram-positive bacteria, they agglutinated only Escherichia coli K-12 and Bacillus subtilis among the bacteria tested. The binding was inhibited by L-rhamnose. STLs bound to distinct serotypes of lipopolysaccharides (LPSs), and showed much higher binding activities to smooth-type LPSs of Escherichia coli K-12 and Shigella flexneri 1A than to their corresponding rough-type LPSs. STLs also bound to lipoteichoic acid (LTA) of Bacillus subtilis. These results indicate that STLs bound to bacteria by recognizing LPSs or LTA on the cell surfaces.