Policy guidelines for the utilization of formalin-fixed, paraffin-embedded tissue sections: the UNCSPORE experience
Policy guidelines for the utilization of formalin-fixed, paraffin-embedded tissue sections: the UNCSPORE experience
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DOI：
10.1023/a:1006354627669
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发表时间：
1999-11-01
影响因子：
3.8
通讯作者：
Newman, B
中科院分区：
文献类型：
--
作者：
Dressler, LG;Geradts, J;Newman, B
石蜡块是一种宝贵的资源，使研究人员能够应用当今的技术，在更短的时间内解决科学问题，并在更多样化的人群中比新鲜或冷冻组织更有可能。然而，除了作为一种可耗尽的资源之外，还存在关于这些组织的适当使用的关注，这既涉及医学或法律的考虑，也涉及质量控制和质量保证实践。我们描述了解决这些问题的政策指导方针，包括：保障措施，以解决医疗/法律的和患者的保密问题，质量控制和质量保证组织切片，处理和存储，数据库管理样本跟踪，并利用标本的科学审查。这些政策和程序是由北卡罗来纳州大学（University of North Carolina）乳腺癌免疫组织化学（IHC）核心实验室的卓越研究专业计划（SPORE）与我们的研究病理学家、受试者和研究者合作制定和实施的。我们希望，这里描述的信息和经验可能会激发讨论，最终导致一个统一的政策，处理福尔马林固定石蜡包埋组织的研究。
Paraffin blocks represent a valuable resource that has allowed investigators to apply today's technology to address scientific questions in a shorter period of time and in more diverse populations than would have been possible with fresh or frozen tissue. However, in addition to being an exhaustible resource, there is concern regarding the appropriate use of these tissues, both with respect to medical or legal considerations and quality control and quality assurance practices. We describe policy guidelines to address these concerns, including: safeguards to address medical/legal and patient confidentiality issues, quality control and quality assurance for tissue sectioning, processing and storage, database management for sample tracking, and scientific review for utilization of specimens. These policies and procedures have been developed and implemented by the University of North Carolina (UNC) Specialized Program of Research Excellence (SPORE) in the Breast Cancer Immunohistochemistry (IHC) Core laboratory, in collaboration with our study pathologists, participants, and research investigators. It is our hope that the information and experience described here may stimulate discussion that can ultimately lead to a uniform policy for handling formalin-fixed paraffin-embedded tissues in research.