Interaction of casein kinase 1 delta (CK1δ) with post-Golgi structures, microtubules and the spindle apparatus
Interaction of casein kinase 1 delta (CK1δ) with post-Golgi structures, microtubules and the spindle apparatus
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DOI：
10.1078/s0171-9335(04)70027-8
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发表时间：
2000-04-01
影响因子：
6.6
通讯作者：
Knippschild, U
中科院分区：
文献类型：
--
作者：
Behrend, L;Stöter, M;Knippschild, U
丝氨酸/苏氨酸激酶的酪蛋白激酶1家族的成员从酵母到哺乳动物是高度保守的，并且似乎在囊泡运输、DNA修复、细胞周期进展和胞质分裂中起重要作用。在这里，我们报告说，在间期细胞的各种哺乳动物物种酪蛋白激酶I δ（CK 1 δ）专门相互作用的反式高尔基体网络和细胞质，颗粒状颗粒，与微管。此外，在有丝分裂时，CK 1 δ被募集到细胞中的纺锤体和中心体，这些细胞已经暴露于DNA损伤剂如依托泊苷或γ-辐射。此外，在免疫沉淀-Western分析中测定CK 1 δ与不同微管蛋白亚型的亲和力揭示了在引入DNA损伤后，CK 1 δ与来自有丝分裂提取物的微管蛋白之间的复合物形成显著增强。在DNA损伤的细胞中，CK 1 δ与纺锤体的高亲和力及其磷酸化几种微管相关蛋白的能力表明了一种调节性。CK 1 δ在有丝分裂中的作用
Members of the casein kinase 1 family of serine/threonine kinases are highly conserved from yeast to mammals and seem to play an important role in vesicular trafficking, DNA repair, cell cycle progression and cytokinesis. We here report that in interphase cells of various mammalian species casein kinase I delta (CK1 delta) specifically interacts with the trans Golgi network and cytoplasmic, granular particles that associate with microtubules. Furthermore, at mitosis CK1 delta is recruited to the spindle apparatus and the centrosomes in cells, which have been exposed to DNA-damaging agents Like etoposide or gamma-irradiation, In addition, determination of the affinity of CK1 delta to different tubulin isoforms in immunoprecipitation-Western anat sis revealed a dramatically enhanced complex formation between CK1 delta and tubulins from mitotic extracts after introducing DNA damage. The high affinity of CK1 delta to the spindle apparatus in DNA-damaged cells and its ability to phosphorylate several microtubule-associated proteins points to a regulatory. role of CK1 delta at mitosis.