Mucosal and systemic immune responses to plasmid protein pgp3 in patients with genital and ocular Chlamydia trachomatis infection
Mucosal and systemic immune responses to plasmid protein pgp3 in patients with genital and ocular Chlamydia trachomatis infection
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DOI：
10.1046/j.1365-2249.2003.02163.x
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发表时间：
2003-06-01
影响因子：
4.6
通讯作者：
Lewis, DJM
中科院分区：
文献类型：
--
作者：
Ghaem-Maghami, S;Ratti, G;Lewis, DJM
使用酶联免疫斑点测定 (ELISPOT) 和 ELISA，对眼部或生殖器衣原体感染的儿童和成人的循环和宫颈 B 细胞对沙眼衣原体质粒蛋白 pgp3 的反应进行了表征。在健康对照中未检测到 pgp3 特异性 ASC，但在患有单纯性宫颈炎或尿道炎的英国成年人中主要检测到 IgA ASC（P = 0.03，0.019）。在患有生殖器外并发症或盆腔炎的患者中，明显存在更多 IgG 和 IgM ASC 的混合反应，表明粘膜免疫区划破坏导致更广泛的感染。在患有衣原体宫颈炎的女性中，就诊时宫颈中存在主要分泌 IgA 和 IgG 的 ASC，其频率比血液高 30-50 倍。抗生素治疗六周后，宫颈 ASC 数量，尤其是 IgG，显着下降。我们主要在冈比亚流行区的儿童中检测到 IgA pgp3 特异性抗体分泌细胞 (ASC)，在严重的沙眼炎症期间，IgA 反应有抑制的趋势 (P = 0.06)，正如之前对其他衣原体抗原的报道，并且与生殖器疾病的发现一致。这些数据为进一步研究衣原体引起的疾病的人类和动物模型中对 pgp3 的免疫反应及其在诊断测定和保护性免疫策略中的潜在用途提供了理论依据。
The circulating and cervical B cell responses to Chlamydia trachomatis plasmid protein pgp3 were characterized in children and adults with ocular or genital chlamydial infection using the enzyme-linked immunospot assay (ELISPOT) and ELISA. No pgp3-specific ASCs were detected in healthy controls, but predominantly IgA ASCs were detected in UK adults with uncomplicated cervicitis or urethritis (P = 0.03, 0.019). In patients with extragenital complications or pelvic inflammatory disease a mixed response with more IgG and IgM ASCs was evident, suggesting a breach of mucosal immune compartmentalization with more extensive infection. In women with chlamydial cervicitis, ASCs secreting predominantly IgA, but also IgG, to pgp3 were present in cervix at presentation, with a frequency 30-50 times higher than blood. Cervical ASC numbers, especially IgG, fell markedly six weeks after antibiotic treatment. We detected principally IgA pgp3-specific antibody secreting cells (ASCs) in children resident in a Gambian endemic area, with a trend towards suppression of IgA responses during intense trachomatous inflammation (P = 0.06), as previously reported for other chlamydial antigens, and in keeping with the findings in genital disease. These data provide a rationale for further studies of immune responses to pgp3 in humans and animal models of chlamydia-induced disease, and its potential use in diagnostic assays and protective immunization strategies.