Endothelin-1 Mediates Prostaglandin F2α-Induced Luteal Regression in the Ewe1
Endothelin-1 Mediates Prostaglandin F2α-Induced Luteal Regression in the Ewe1
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Endothelin-1 介导前列腺素 F2α 诱导的 Ewe1 黄体退化
DOI：
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
R. Milvae
中科院分区：
文献类型：
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作者：
S. Hinckley;R. Milvae
摘要通过一系列实验研究内皮素-1 （ET-1）在羊黄体功能中的潜在作用。在2小时的孵育过程中，内皮素-1抑制分散的羊黄体细胞产生基底和lh刺激的黄体酮。当细胞与高特异性内皮素- ETA受体拮抗剂环-d- asp - pro -d- val - leu -d- trp （BQ123）预孵育时，这种抑制作用被消除。在周期中期收集的羊黄体（CL）中，给予促黄体素F2α (PGF2α)快速刺激ET-1基因表达。母羊在发情周期的第8天或第9天静脉注射单剂量BQ123可减轻PGF2α的溶血作用。母羊在月经中期肌注100 μg ET-1可降低其余发情周期的血浆孕酮浓度。经半溶性PGF2α预处理后，ig 100 μg ET-1可使血浆孕酮迅速下降，缩短发情周期。这些数据补充和扩展了先前发表的关于牛CL的报告，是迄今为止提出的支持ET-1在pgf2 α-介导的家养反刍动物黄体功能中作用的最有力证据。
Abstract A diversified series of experiments was conducted to determine the potential role of endothelin-1 (ET-1) in ovine luteal function. Endothelin-1 inhibited basal and LH-stimulated progesterone production by dispersed ovine luteal cells during a 2-h incubation. This inhibition was removed when cells were preincubated with cyclo-d-Asp-Pro-d-Val-Leu-d-Trp (BQ123), a highly specific endothelin ETA receptor antagonist. Administration of a luteolytic dose of prostaglandin F2α (PGF2α) rapidly stimulated gene expression for ET-1 in ovine corpora lutea (CL) collected at midcycle. Intraluteal administration of a single dose of BQ123 to ewes on Day 8 or 9 of the estrous cycle mitigated the luteolytic effect of PGF2α. Intramuscular administration of 100 μg ET-1 to ewes at midcycle reduced plasma progesterone concentrations for the remainder of the estrous cycle. Following pretreatment with a subluteolytic dose of PGF2α, i.m. administration of 100 μg ET-1 caused a rapid decline in plasma progesterone and shortened the length of the estrous cycle. These data complement and extend previously published reports in the bovine CL and are the strongest evidence presented to date in support of a role for ET-1 in PGF2α-mediated luteal function in domestic ruminants.