Electrochemical functionalization of polypyrrole nanowires for the development of ultrasensitive biosensors for detecting microRNA
Electrochemical functionalization of polypyrrole nanowires for the development of ultrasensitive biosensors for detecting microRNA
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DOI：
10.1016/j.snb.2018.10.131
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发表时间：
2019-02
期刊：
影响因子：
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通讯作者：
Jiasheng Wang;N. Hui
中科院分区：
文献类型：
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作者：
Jiasheng Wang;N. Hui
以聚乙二醇（PEG）-聚吡咯（PPy）纳米线为基底，采用电化学图案化技术制备超灵敏生物传感器。采用恒电位电化学聚合法在玻碳电极表面制备了聚吡咯纳米线阵列。聚吡咯纳米线表面修饰与4臂PEG分子通过电化学氧化的胺基提供的PEG末端链。所制备的PEG/PPy纳米线综合了导电聚合物PPy纳米线的优良导电性能和PEG的良好介电性能。microRNAs（miRNAs）在肿瘤的发生、发展和多种疾病的发生、发展中起着重要作用，是研究疾病发生、预后和风险的生物标志物。因此，建立疾病的miRNA谱和检测生物样品中的miRNA是诊断学中的关键里程碑。将DNA探针固定在聚乙二醇/聚吡咯纳米线上，可以方便地制备一种超灵敏的miRNA电化学生物传感器。用微分脉冲伏安法（DPV）检测亚甲基蓝（MB）氧化还原信号的变化来监测DNA/RNA杂交。该生物传感器对靶miRNA的线性范围较宽（0.10 pM ~ 1.0 nM），且对错配的miRNA也能很好地识别。此外，基于PEG/PPy纳米线的生物传感器可以扩展到任何类型的基于DNA的生物传感器的开发。
A simple electrochemical patterning strategy has been developed for the construction of ultrasensitive biosensors based on polyethylene glycol (PEG)-polypyrrole (PPy) nanowires substrate in order to supply improved antifouling performances. PPy nanowires array was produced through electrochemical polymerization of pyrrole at constant potential onto glassy carbon electrode (GCE) surface. PPy nanowires surfaces were decorated with 4-armed PEG molecules by electrochemical oxidation of amine groups offered by PEG end chains. The prepared PEG/PPy nanowires have integrated the excellent electrical conductivity of conducting polymer PPy nanowires with the good antifouling property of PEG. MicroRNAs (miRNAs) play very important roles in cancer development and a variety of diseases, which make them become promising biomarkers of the onset, prognosis and risk of diseases. Therefore the establishment of miRNA profiles for diseases and the detection of miRNAs in biological samples are critical milestones in diagnostics. An ultrasensitive electrochemical biosensor for miRNA can be effortlessly developed by the immobilization of DNA probes onto PEG/PPy nanowires. DNA/RNA hybridization was monitored by changes of methylene blue (MB) redox signal using differential pulse voltammetry (DPV) method. A wide linear range (0.10 pM ∼ 1.0 nM) to target miRNA was obtained by the fabricated biosensor, and miRNA mismatches can also be easily identified with satisfactory. Furthermore, the biosensor based on PEG/PPy nanowires can be extended to the development of any type of DNA-based biosensor.