Arabidopsis ABCG14 protein controls the acropetal translocation of root-synthesized cytokinins
Arabidopsis ABCG14 protein controls the acropetal translocation of root-synthesized cytokinins
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DOI：
10.1038/ncomms4274
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发表时间：
2014-02
影响因子：
16.6
通讯作者：
Kewei Zhang;O. Novák;Zhaoyang Wei;Mingyue Gou;Xuebin Zhang;Yong Yu;Huijun Yang;Yuanheng Cai;M. Strnad;Chang‐Jun Liu
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文献类型：
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作者：
Kewei Zhang;O. Novák;Zhaoyang Wei;Mingyue Gou;Xuebin Zhang;Yong Yu;Huijun Yang;Yuanheng Cai;M. Strnad;Chang‐Jun Liu
细胞分裂素是调节植物生长发育和逆境反应的一类重要激素。然而，与生长素的极性运输相反，细胞分裂素运输的分子基础知之甚少。我们发现，拟南芥中的ATP结合盒转运蛋白AtABCG 14是根合成的细胞分裂素向顶（根到地上部）转运所必需的，AtABCG 14主要在根的周鞘和中柱细胞中表达。AtABCG14基因的敲除会严重影响玉米素（tZ）型细胞分裂素从根向芽的转运，从而影响植物的生长发育。AtABCG 14定位于转化细胞的质膜上，C14或C13标记的dtZ在植物中的摄食表明它作为外排泵，其在细胞中的存在与摄食细胞分裂素的转运直接相关。因此，AtABCG 14是一种可能参与植物细胞分裂素长距离转运的转运蛋白。
Cytokinins are a major group of phytohormones regulating plant growth, development and stress responses. However, in contrast to the well-defined polar transport of auxins, the molecular basis of cytokinin transport is poorly understood. Here we show that an ATP-binding cassette transporter inArabidopsis, AtABCG14, is essential for the acropetal (root to shoot) translocation of the root-synthesized cytokinins.AtABCG14is expressed primarily in the pericycle and stelar cells of roots. Knocking outAtABCG14strongly impairs the translocation oftrans-zeatin (tZ)-type cytokinins from roots to shoots, thereby affecting the plant’s growth and development. AtABCG14 localizes to the plasma membrane of transformed cells.In plantafeeding of C14or C13-labelledtZ suggests that it acts as an efflux pump and its presence in the cells directly correlates with the transport of the fed cytokinin. Therefore, AtABCG14 is a transporter likely involved in the long-distance translocation of cytokininsin planta.