Lipoprotein lipase distribution in rat adipose tissues: effect on chylomicron uptake.
Lipoprotein lipase distribution in rat adipose tissues: effect on chylomicron uptake.
复制标题
大鼠脂肪组织中脂蛋白脂肪酶的分布：对乳糜微粒摄取的影响。
DOI：
10.1152/ajpendo.1977.232.3.e316
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发表时间：
1977
期刊：
影响因子：
--
通讯作者：
A. D. Hartman
中科院分区：
文献类型：
--
作者：
A. D. Hartman
在50 ~ 550 g体重范围内，大鼠附睾（Epi）和肾周（PR）贮库中的脂肪细胞不断增大。相比之下，皮下（SC）和肠系膜（M）贮库中的细胞大小仅在大鼠达到250-350 g之前与其他两个贮库中的细胞大小相似。此后，脂肪细胞在后者仓库显着小于前者。在所有四个贮库中，每10（6）个细胞的脂蛋白脂酶（LPL）活性随着进食和禁食大鼠体型的增加而增加。在每10（6）个细胞中的LPL活性在两种营养状态下作为细胞大小的函数在贮库之间没有显著差异。在生长过程中，所有贮库的全组织LPL的更大百分比被隔离在扩大的脂肪细胞内，导致细胞外活性的绝对下降。这种影响在肾上腺素和PR仓库中最不明显。在大的脂肪细胞喂养大鼠，减少细胞外定位LPL在体内减少乳糜微粒甘油三酯脂肪酸的摄取在所有的仓库。在禁食大鼠中，摄取较低，并且不受所有部位细胞大小变化的影响。
Adipocytes in the epididymal (Epi) and perirenal (PR) depots of the rat increased continuously in size in rats ranging fromm 50 to 550 g body wt. In contrast, cell size in the subcutaneous (SC) and mesenteric (M) depots was similar to that in the other two depots only until rats achieved 250-350 g. Thereafter, adipocytes in the latter depots were significantly smaller than in the former. In all four depots, the activity of lipoprotein lipase (LPL) per 10(6) cells increased with increasing size in both fed and fasted rats. No significant differences were noted in LPL activity per 10(6) cells between depots as a function of cell size in either nutritional state. During growth, a greater percentage of whole tissue LPL of all depots was sequestered within enlarging adipocytes, resulting in an absolute decrease in activity extracellulalrly. This effect was least pronounced in the Epi and PR depots. In large adipocytes of fed rats, decreased extracellular localization of LPL was paralleled in vivo by decreased uptake of chylomicron triglyceride fatty acids in all depots. In fasted rats, uptake was low and unaffected by changes in cell size in all sites.