Opposite variations in fumarate and malate dominate metabolic phenotypes of Arabidopsis salicylate mutants with abnormal biomass under chilling.
Opposite variations in fumarate and malate dominate metabolic phenotypes of Arabidopsis salicylate mutants with abnormal biomass under chilling.
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DOI：
10.1111/ppl.12210
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发表时间：
2014-12
影响因子：
6.4
通讯作者：
I. Scott;J. Ward;Sonia J. Miller;M. H. Beale
中科院分区：
文献类型：
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作者：
I. Scott;J. Ward;Sonia J. Miller;M. H. Beale
在低温(5℃)条件下，水杨酸(SA)缺乏突变体(sid2、eds5和NahG)比野生型(Col-0)产生更多的生物量，而SA高产的cpr1受到极大的抑制。利用600 MHz(1)H核磁共振(核磁共振)分析，对极地上部提取物进行了分析，探讨了这些表型在新陈代谢中的反映。生物量相关的代谢表型被确认为这些核磁共振“指纹”的多变量数据模型。其中包括与生物量相关的主要成分。此外，偏最小二乘回归模型还发现，在以前未见过的不同光照强度的实验中，偏最小二乘回归模型可以预测植物的相对大小，或者预测一个基因与其他基因的相对大小。在这些模型中，富马酸是主导信号，在SA缺乏突变体中含量较高，在Col-0中居中，在cpr1中含量较低。在与生物量负相关的信号中，苹果酸占主导地位。FUM2胞质延胡索酸酶(At5g50950)转录本的丰度与富马酸水平和生物量呈显著正相关，而FUM1线粒体延胡索酸酶(At2g47510)转录本的丰度与富马酸水平和生物量无显著差异。证实了SA在低温下的形态效应在代谢组中表达，其中富马酸的作用尤为突出。
In chilling conditions (5°C), salicylic acid (SA)-deficient mutants (sid2, eds5 and NahG) of Arabidopsis thaliana produced more biomass than wild type (Col-0), whereas the SA overproducer cpr1 was extremely stunted. The hypothesis that these phenotypes were reflected in metabolism was explored using 600 MHz (1) H nuclear magnetic resonance (NMR) analysis of unfractionated polar shoot extracts. Biomass-related metabolic phenotypes were identified as multivariate data models of these NMR 'fingerprints'. These included principal components that correlated with biomass. Also, partial least squares-regression models were found to predict the relative size of plants in previously unseen experiments in different light intensities, or relative size of one genotype from the others. The dominant signal in these models was fumarate, which was high in SA-deficient mutants, intermediate in Col-0 and low in cpr1 at 5°C. Among signals negatively correlated with biomass, malate was prominent. Abundance of transcripts of the FUM2 cytosolic fumarase (At5g50950) showed strong positive correlation with fumarate levels and with biomass, whereas no significant differences were found for the FUM1 mitochondrial fumarase (At2g47510). It was confirmed that the morphological effects of SA under chilling find expression in the metabolome, with a role of fumarate highlighted.