[Effect of electroacupuncture intervention at different time-points of post-modeling on hippocampal monoamine neurotransmitter levels in mice with vascular dementia].
[Effect of electroacupuncture intervention at different time-points of post-modeling on hippocampal monoamine neurotransmitter levels in mice with vascular dementia].
复制标题
DOI：
--
复制
发表时间：
2014-04
期刊：
影响因子：
--
通讯作者：
Hui-zhen Zhang;Yu-lei Liang;Xue-jing Zhang;Chuang Zhang;Yan-hui Sun;Xiufeng Zhang;Xiao-kang Xu;Rui-Guo Jia;Xin-Guo Wang
中科院分区：
文献类型：
--
作者：
Hui-zhen Zhang;Yu-lei Liang;Xue-jing Zhang;Chuang Zhang;Yan-hui Sun;Xiufeng Zhang;Xiao-kang Xu;Rui-Guo Jia;Xin-Guo Wang
目的观察造模后不同时间点电针干预对血管性痴呆（VD）小鼠行为学及海马单胺类神经递质去甲肾上腺素（NE）、多巴胺（DA）、5-羟色胺（5-HT）含量的影响，探讨其改善VD的机制。方法昆明种小鼠60只，随机分为假手术对照组（n = 20）、VD模型组（n = 20）、EA-d（D）-1组（从造模后第1天开始电针，n = 10）、EA-d-3组（从造模后第3天开始电针，n = 10）。采用双侧颈总动脉阻断再灌注的方法建立VD模型。电针（2Hz/80Hz）"百会"、"大椎"、"足三里"、"膈俞"穴10min，每日1次，连续15d。荧光分光光度法测定海马NE、DA和5-HT含量。通过跳台实验评估小鼠的学习记忆能力。结果与假手术对照组比较，模型组大鼠学习记忆能力明显降低（反应时、错误次数明显增加，跳台潜伏期明显缩短）（P <0.01）。模型组海马NE、DA、5-HT含量明显低于假手术组（P <0.01）。与模型组相比，电针干预组反应时、错误次数明显减少，跳台潜伏期明显延长海马NE、DA、5-HT含量显著升高（P <0.01）EA-D-3组疗效明显优于EA-D-1组（P <0.05，P <0.01）。结论电针能改善VD小鼠的学习记忆能力，这与其上调海马NE、DA和5-HT含量密切相关，且在造模后再行电针干预效果更好。
OBJECTIVE To observe the effect of electrocupuncture (EA) intervention at different time-points of post-modeling on behavior and hippocampal monoamine neurotransmitter noradrenalin (NE), dopamine (DA) and 5-hydroxytrypamine (5-HT) contents in vascular dementia (VD) mice, so as to study its mechanism underlying improvement of VD. METHODS A total of 60 Kunming mice were randomized into sham-operation control (n = 20), VD model (n = 20), EA-day (D)-1 (EA treatment was given from the 1st day on after modeling, n = 10), EA-D-3 (EA was given from the 3rd day on after modeling, n = 10) groups. VD model was established by occlusion of the bilateral cervical common arteries and reperfusion. EA (2 Hz/80 Hz) was applied to "Baihui" (GV 20), "Dazhui" (GV 14),"Zusanli" (ST 36) and "Geshu"(BL 17) for 10 min, once daily for 15 days. Hippocampal NE, DA and 5-HT contents were assayed by fluorospectrophotometry. The mouse's learning-memory ability was assessed by step-down tests. RESULTS In comparison with the sham-operation control group, the learning-memory ability (marked increase of reaction time and error times, decrease of step-down latency) was apparently lowered in the model group (P < 0.01). The hippocampal NE, DA and 5-HT contents were significantly lower in the model group than in the sham-operation group (P < 0.01). Compared with the model group, the mice's learning-memory ability (marked decrease of reaction time and error times, increase of step-down latency) was significantly increased in EA intervention groups (P < 0.01), and hippocampal NE, DA and 5-HT levels were significantly increased (P < 0.01), and the effect of EA-D-3 group was obviously better than that of the EA-D-1 group (P < 0.05, P < 0.01). CONCLUSION EA can improve the VD mice's learning-memory ability, which is closely related to its effects in up-regulating hippocampal NE, DA and 5-HT contents, and the effect of later EA intervention after modeling is better.