Intranasal immunization with formalin-inactivated virus vaccine induces a broad spectrum of heterosubtypic immunity against influenza A virus infection in mice
Intranasal immunization with formalin-inactivated virus vaccine induces a broad spectrum of heterosubtypic immunity against influenza A virus infection in mice
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DOI：
10.1016/s0264-410x(03)00234-2
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发表时间：
2003-07-04
期刊：
影响因子：
5.5
通讯作者：
Kida, H
中科院分区：
文献类型：
--
作者：
Takada, A;Matsushita, S;Kida, H
已知甲型流感病毒感染诱导具有不同亚型病毒包膜蛋白、血凝素（HA）和神经氨酸酶（NA）的病毒感染的交叉保护性免疫。这种异亚型免疫通常是由细胞毒性T淋巴细胞（CTL）介导的，CTL对病毒内部蛋白质（如核蛋白和基质蛋白）中的特定表位有反应。相比之下，灭活病毒抗原免疫被认为不能产生异亚型免疫，因为灭活抗原通常不会诱导CTL反应。然而，我们发现，用福尔马林灭活的完整病毒鼻内免疫，而不是用醚分裂疫苗，在小鼠中诱导了广谱的异亚型保护性免疫。这种保护可能是由粘膜免疫反应介导的，最可能的是分泌针对病毒蛋白的IgA抗体。这种方法可以克服灭活流感疫苗效力的局限性，使人类对具有新的大流行潜力的病毒具有强大的免疫力。2003爱思唯尔科学有限公司版权所有。
It has been known that influenza A virus infection induces a cross-protective immunity against infection by viruses with different subtypes of viral envelope proteins, hemagglutinin (HA) and neuraminidase (NA). This heterosubtypic immunity is generally mediated by cytotoxic T lymphocytes (CTL) reactive to specific epitopes in the viral internal proteins, such as nucleoprotein and matrix protein. By contrast, immunization with inactivated virus antigens has been thought to be unable to generate heterosubtypic immunity, since inactivated antigens do not usually induce CTL responses. However, we show that intranasal immunization with formalin-inactivated intact virus, but not ether-split vaccines, induced a broad spectrum of heterosubtypic protective immunity in mice. The protection may be mediated by the mucosal immune response, most likely secretory IgA antibodies to the viral proteins. This approach may overcome limitations in the efficacy of inactivated influenza vaccines and confer potent immunity to humans against viruses with new pandemic potential. (C) 2003 Elsevier Science Ltd. All rights reserved.